Wang Kunmei, Wang Siyi, Wang Ting, Xia Qi, Xia Shitou
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
J Fungi (Basel). 2023 Dec 25;10(1):12. doi: 10.3390/jof10010012.
The ADP-ribosylation factor 6 (Arf6), as the only member of the Arf family III protein, has been extensively studied for its diverse biological functions in animals. Previously, the Arf6 protein in was found to be crucial for endocytosis and polarity establishment during asexual development. However, its role remains unclear in . Here, we identified and characterized in using a reverse genetic approach. Deletion of impaired hyphal growth and development and produced more branches. Interestingly, knockout of resulted in an augmented tolerance of towards oxidative stress, and increased its sensitivity towards osmotic stress, indicative of the different roles of in various stress responses. Simultaneously, deletion led to an elevation in melanin accumulation. Moreover, the appressorium formation was severely impaired, and fungal virulence to host plants was significantly reduced. Overall, our findings demonstrate the essential role of in hyphal development, stress responses, appressorium formation, and fungal virulence to host plants.
ADP核糖基化因子6（Arf6）作为Arf家族III蛋白的唯一成员，因其在动物体内多样的生物学功能而受到广泛研究。此前，已发现其Arf6蛋白在无性发育过程中的内吞作用和极性建立中至关重要。然而，其在……中的作用仍不清楚。在此，我们采用反向遗传学方法在……中鉴定并表征了……。……的缺失损害了菌丝生长和发育，并产生了更多分支。有趣的是，……的敲除导致……对氧化应激的耐受性增强，而对渗透应激的敏感性增加，这表明……在各种应激反应中具有不同作用。同时，……的缺失导致黑色素积累增加。此外，附着胞的形成严重受损，并且真菌对寄主植物的毒力显著降低。总体而言，我们的研究结果证明了……在菌丝发育、应激反应、附着胞形成以及真菌对寄主植物的毒力方面的重要作用。
