Hwang Ji-Yeon, Kim Jung-Eun, Song Yoon-Jae, Park Jong-Hyun
1Department of Food Science and Biotechnology, Gachon University, Seongnam, Gyeonggi, 13120 Korea.
2Department of Life Science, Gachon University, Seongnam, Gyeonggi, 13120 Korea.
Food Sci Biotechnol. 2016 Feb 29;25(1):355-360. doi: 10.1007/s10068-016-0050-6. eCollection 2016.
Use of bacteriophages as sanitizing agents has received much attention. However, safety in humans is debatable. To determine inflammatory immune responses against bacteriophages, rats were treated with a 8 log plaque-forming cocktail of 5 bacteriophages for pathogenic per day for 4 weeks. Food consumption, feeding efficiency, and body weight of rats treated with the cocktail were not different from controls. Phages were not detected in the sera of phage-fed rats with no changes in organ weights. Notable changes were not observed upon histopathological examination of the liver, kidney, and spleen. Pro-inflammatory cytokine mRNA expression, except COX-2 (2.4x increase), remained unaffected after treatment with the phage cocktail. No remarkable changes were observed for levels of 12 pro-inflammatory cytokines in sera. Inflammatory responses in rats orally treated with a phage cocktail were not observed. Bacteriophages for are indicated as immunologically safe in rats.
将噬菌体用作消毒剂已备受关注。然而，其对人类的安全性存在争议。为确定针对噬菌体的炎性免疫反应，用含5种噬菌体的8个对数噬菌斑形成混合物对大鼠进行处理，以用于致病性治疗，每天一次，持续4周。用该混合物处理的大鼠的食物消耗、进食效率和体重与对照组无异。在喂食噬菌体的大鼠血清中未检测到噬菌体，器官重量也无变化。对肝脏、肾脏和脾脏进行组织病理学检查时未观察到明显变化。用噬菌体混合物处理后，除COX - 2（增加2.4倍）外，促炎细胞因子mRNA表达未受影响。血清中12种促炎细胞因子水平未观察到显著变化。未观察到口服噬菌体混合物的大鼠出现炎症反应。表明用于[此处原文缺失相关内容]的噬菌体在大鼠中具有免疫安全性。
