发芽糯黑米提取物通过调节3T3-L1脂肪细胞中多个基因的表达来抑制脂质积累。
Germinated waxy black rice extract inhibits lipid accumulation with regulation of multiple gene expression in 3T3-L1 adipocytes.
作者信息
Lim Won Chul, Ho Jin Nyoung, Lee Hee Seop, Cho Hong Yon
机构信息
2Department of Food and Biotechnology, Korea University, Sejong, 30019 Korea.
1Department of Medical Nutrition, Kyung Hee University, Yongin, Gyeonggi, 17104 Korea.
出版信息
Food Sci Biotechnol. 2016 Jun 30;25(3):821-827. doi: 10.1007/s10068-016-0137-0. eCollection 2016.
The aim of this study was to investigate the anti-obesity effects of germinated waxy black rice (GWBR) extract in 3T3-L1 adipocytes. The inhibitory effect of GWBR extract against adipocyte differentiation was evaluated using Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) assay. GWBR extract inhibited adipocyte differentiation, but was not found to elicit any cytotoxicity. The mRNA levels of adipogenic transcriptional factors such as C/EBP-α and -β, PPAR-γ, and SREBP-1c, as well as adipogenic enzymes, including aP2, LPL, and FAS were significantly downregulated by treatment with GWBR extract compared to untreated control cells. However, mRNA levels of lipolytic genes such as HSL and ATGL, β-oxidation related genes CPT1, and UCP2 involved in thermogenesis were significantly up-regulated by treatment with GWBR extract. These data suggest that GWBR extract may be a potential functional food, and may have pharmacological applications in both the prevention and treatment of obesity.
本研究旨在探讨发芽糯黑米（GWBR）提取物对3T3-L1脂肪细胞的抗肥胖作用。采用油红O染色和甘油-3-磷酸脱氢酶（GPDH）测定法评估GWBR提取物对脂肪细胞分化的抑制作用。GWBR提取物抑制脂肪细胞分化，但未发现有任何细胞毒性。与未处理的对照细胞相比，用GWBR提取物处理后，脂肪生成转录因子如C/EBP-α和-β、PPAR-γ和SREBP-1c以及脂肪生成酶（包括aP2、LPL和FAS）的mRNA水平显著下调。然而，用GWBR提取物处理后，脂解基因如HSL和ATGL、参与产热的β-氧化相关基因CPT1和UCP2的mRNA水平显著上调。这些数据表明，GWBR提取物可能是一种潜在的功能性食品，在肥胖症的预防和治疗方面可能具有药理学应用价值。
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