从韩国各种食品中分离出的[未提及具体内容]的随机扩增多态性DNA分型。
RAPD typing of isolated from various food products from Korea.
作者信息
Sharma Anshul, Kaur Jasmine, Lee Sulhee, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, Gyeonggi, 13120 Korea.
出版信息
Food Sci Biotechnol. 2016 Dec 31;25(6):1651-1655. doi: 10.1007/s10068-016-0254-9. eCollection 2016.
Abstract
In the present study, the fingerprinting technique, random amplified polymorphic DNA-PCR was evaluated to characterize 13 strains of , isolated from different vegetable products of South Korea. Two primers i.e. 239 and KAY3 were used. The primer 239 produced bands ranged from 500-4,000 bp and KAY3 primer produced bands with sizes from 600-4,000 bp. Both primers produced thirteen different RAPD profiles. Phylogenetic dendrogram showed that all the isolates could be divided into six major clusters both the primers. However, a few strains of had similar profiles and were not well differentiated by RAPD.
摘要
在本研究中，对随机扩增多态性DNA-PCR指纹技术进行了评估，以鉴定从韩国不同蔬菜产品中分离出的13株[具体菌株名称未给出]。使用了两种引物，即239和KAY3。引物239产生的条带范围为500 - 4000 bp，引物KAY3产生的条带大小为600 - 4000 bp。两种引物均产生了13种不同的RAPD图谱。系统发育树状图显示，两种引物的所有分离株均可分为六个主要聚类。然而，有几株[具体菌株名称未给出]具有相似的图谱，无法通过RAPD很好地区分。
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