Daud Khaled A K, Neilan B A, Henriksson A, Conway P L
School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.
FEMS Microbiol Lett. 1997 Aug 1;153(1):191-7. doi: 10.1111/j.1574-6968.1997.tb10481.x.
Multiplex RAPD-PCR was used to generate unique and identifying DNA profiles for isolates of the genus Lactobacillus. The method that was used was based on the combination of two 10-mer oligonucleotides in a single PCR. The generated RAPD profiles enabled discrimination of all lactobacillus strains that were used in this study. A dendrogram was generated from the RAPD profiles. The results of genetic relatedness obtained from the dendrogram were compared with the results obtained using carbohydrate fermentation profiles. Most of the gastrointestinal isolates studied could not be grouped using carbohydrate fermentation profiles. The RAPD profiles provided sufficient information to prepare a dendrogram of genetic relatedness. The gastrointestinal isolates were clustered together on the dendrogram. Furthermore an isolate originating from the stomach (strain ML004) was closely related to Lactobacillus fermentum. It was concluded that multiplex RAPD-PCR was useful for characterisation and inference of relatedness of Lactobacillus isolates.
多重随机扩增多态性DNA聚合酶链反应（Multiplex RAPD-PCR）被用于生成乳酸杆菌属分离株独特的、可识别的DNA图谱。所使用的方法基于在单个聚合酶链反应中两种10聚体寡核苷酸的组合。生成的随机扩增多态性DNA（RAPD）图谱能够区分本研究中使用的所有乳酸杆菌菌株。从RAPD图谱生成了一个树形图。将从树形图获得的遗传相关性结果与使用碳水化合物发酵图谱获得的结果进行比较。大多数所研究的胃肠道分离株不能用碳水化合物发酵图谱进行分组。RAPD图谱提供了足够的信息来制备遗传相关性树形图。胃肠道分离株在树形图上聚集在一起。此外，一株源自胃部的分离株（菌株ML004）与发酵乳杆菌密切相关。得出的结论是，多重RAPD-PCR对于乳酸杆菌分离株的特征鉴定和相关性推断是有用的。
