Beckman W C, Powers S K, Brown J T, Gillespie G Y, Bigner D D, Camps J L
Cancer. 1987 Jan 15;59(2):266-70. doi: 10.1002/1097-0142(19870115)59:2<266::aid-cncr2820590215>3.0.co;2-6.
The time course of uptake, retention and clearance of the cationic lipophilic dye, rhodamine 123 (Rh123), within the central nervous system was qualitatively evaluated in rats. Weanling rats were injected intracerebrally with avian sarcoma virus, which induced malignant gliomas in situ before injection of Rh123. Comparison was made of the amount of fluorescence of Rh123 within the normal cerebral cortex, myelinated tracts of the brain, meninges, choroid plexus, and neoplastic foci at 1, 4, 8, 12 and 24 hours after intravenous injection. Fluorescence microscopy was utilized to identify tissues containing the dye. Normal neuropil did not contain Rh123 at any of the time periods studied. Gliomas retained the dye at 1, 4, 8 and 12 hours, with increasing uniformity of distribution and decreasing intensity of fluorescence over this time period. Fluorescence was not detected at 24 hours within the neoplastic tissues, but was evident at all time periods studied within the choroid plexus. The specific retention of Rh123 by malignant glioma and by the choroid plexus in vivo suggests that Rh123 may be useful for photochemotherapeutic treatment of brain neoplasms and disorders of the choroid plexus.
在大鼠体内对阳离子亲脂性染料罗丹明123（Rh123）在中枢神经系统内的摄取、滞留和清除的时间进程进行了定性评估。将断奶大鼠脑内注射禽肉瘤病毒，该病毒在注射Rh123之前原位诱导恶性胶质瘤。比较静脉注射后1、4、8、12和24小时正常大脑皮质、脑有髓神经纤维束、脑膜、脉络丛和肿瘤灶内Rh123的荧光量。利用荧光显微镜识别含有该染料的组织。在所研究的任何时间段内，正常神经纤维均不含Rh123。胶质瘤在1、4、8和12小时时保留该染料，在此时间段内分布均匀性增加，荧光强度降低。在24小时时肿瘤组织内未检测到荧光，但在脉络丛内所有研究时间段均可见荧光。恶性胶质瘤和脉络丛在体内对Rh123的特异性保留表明，Rh123可能对脑肿瘤和脉络丛疾病的光化学治疗有用。
