Yamasaki K, Konno T, Miyauchi Y, Maeda H
Cancer Res. 1987 Feb 1;47(3):852-5.
We studied a prophylactic chemotherapy against hepatic metastases arising from the shedding of tumor cells into the portal circulation. The therapy was done with a lymphographic oily contrast medium, Lipiodol, and a high molecular weight anticancer agent named poly(styrene-maleic acid) copolymer conjugated neocarzinostatin (SMANCS), developed in our laboratory. SMANCS was dissolved in Lipiodol by sonication (SMANCS/Lipiodol, 1 mg of SMANCS in 1 ml of Lipiodol). Twelve rabbits were simply inoculated with the highly malignant carcinoma VX-2. Fifteen rabbits were given injections of SMANCS in glucose and Lipiodol into the portal vein and were subsequently inoculated with the tumor cells. Eighteen were given injections of SMANCS/Lipiodol and then the tumor cells. These rabbits were killed 12 days later. Thirteen were given injections of the tumor cells alone and were allowed to survive. Sixteen were given injections of SMANCS/Lipiodol and then with the tumor cells; they were allowed to survive. Rabbits given injections of SMANCS/Lipiodol before tumor inoculation had significantly fewer (P less than 0.001) metastases than those not treated or those given SMANCS in glucose and Lipiodol. Survival was significantly longer [P less than 0.005; 36.0 +/- 7.7 (SD) days] with SMANCS/Lipiodol before tumor inoculation than without treatment [23.5 +/- 3.0 days]. SMANCS/Lipiodol has a prolonged anticancer effect because it remains in the portal vein and allows sustained drug release from the oil (Lipiodol) to aqueous spaces. Hepatic metastases might be prevented by portal administration of the appropriate oily anticancer agent.
我们研究了一种针对肿瘤细胞脱落进入门静脉循环而引发肝转移的预防性化疗方法。该治疗使用的是一种淋巴造影油性造影剂——碘油，以及我们实验室研发的一种名为聚（苯乙烯 - 马来酸）共聚物偶联新制癌菌素（SMANCS）的高分子量抗癌剂。通过超声处理将SMANCS溶解于碘油中（SMANCS/碘油，每1毫升碘油中含1毫克SMANCS）。12只兔子单纯接种高恶性VX - 2癌。15只兔子经门静脉注射葡萄糖和碘油中的SMANCS，随后接种肿瘤细胞。18只兔子注射SMANCS/碘油，然后接种肿瘤细胞。12天后处死这些兔子。13只兔子仅注射肿瘤细胞并任其存活。16只兔子注射SMANCS/碘油，然后接种肿瘤细胞，任其存活。在肿瘤接种前注射SMANCS/碘油的兔子发生的转移灶明显少于未治疗的兔子或注射葡萄糖和碘油中SMANCS的兔子（P小于0.001）。肿瘤接种前注射SMANCS/碘油的兔子的存活时间明显更长[P小于0.005；36.0±7.7（标准差）天]，而未治疗的兔子存活时间为[23.5±3.0天]。SMANCS/碘油具有延长的抗癌作用，因为它留存于门静脉中，能使药物从油（碘油）持续释放到水相空间。通过门静脉给予合适的油性抗癌剂可能预防肝转移。
