Department Of Chemistry, Khouzestan Science and Research Branch, Islamic Azad University, Ahvaz, Iran.
Department Of Chemistry, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
J Sci Food Agric. 2019 Mar 15;99(4):1919-1925. doi: 10.1002/jsfa.9387. Epub 2018 Nov 28.
Malachite green is used in aquaculture and fisheries as a fungicide and antiseptic and it is also used in industry as a dye. However, malachite green is carcinogenic and highly toxic for humans and animals. In this study, a spectrophotometric method was developed to detect malachite green. The method was based on the surface plasmon resonance property of gold nanoparticles and interaction between malachite green and gold nanoparticles.
Malachite green-gold nanoparticles were rapidly aggregated in the acidic medium; as a result, a color change from red to blue was observed, which was easily detectable by the naked eye. The absorption ratio (A623/A520) of the gold nanoparticles in an optimized system exhibited a linear correlation with malachite green concentration. The method detection limit and linear range were 3 and 50-350 ng mL , respectively. The method was applied successfully to detect malachite green in different samples.
The method was simple and rapid to detect malachite green. The most important advantages of the method are the possibility of malachite green determination with very good accuracy and sensitivity using a simple UV-visible spectrometer without any expensive or sophisticated instrumentation and also the versatility of real samples. © 2018 Society of Chemical Industry.
孔雀石绿在水产养殖和渔业中用作杀菌剂和防腐剂，在工业中也用作染料。然而，孔雀石绿对人类和动物具有致癌性和高度毒性。在这项研究中，开发了一种分光光度法来检测孔雀石绿。该方法基于金纳米粒子的表面等离子体共振特性以及孔雀石绿与金纳米粒子之间的相互作用。
孔雀石绿-金纳米粒子在酸性介质中迅速聚集；因此，观察到颜色从红色变为蓝色，这可以用肉眼轻易检测到。在优化体系中，金纳米粒子的吸收比（A623/A520）与孔雀石绿浓度呈线性相关。该方法的检测限和线性范围分别为 3 和 50-350ng mL 。该方法成功应用于不同样品中孔雀石绿的检测。
该方法简单快速，可用于检测孔雀石绿。该方法最重要的优点是，使用简单的紫外-可见分光光度计，可以非常准确和灵敏地测定孔雀石绿，而无需任何昂贵或复杂的仪器，并且还可以用于实际样品的通用性。© 2018 化学工业协会。
