Aïnad-Tabet S, Grar H, Haddi A, Negaoui H, Guermat A, Kheroua O, Saïdi D
Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Natural and Life Sciences, University of Oran1 Ahmed Ben Bella, 31000 Oran, Algeria.
Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Natural and Life Sciences, University of Oran1 Ahmed Ben Bella, 31000 Oran, Algeria.
Allergol Immunopathol (Madr). 2019 May-Jun;47(3):214-220. doi: 10.1016/j.aller.2018.07.010. Epub 2018 Sep 27.
Allergy to cow's milk proteins has often been associated with dysfunction of the intestinal mucosa caused by chronic inflammation in infants. This study evaluated the protective effect of taurine on intestinal damage induced by beta-lactoglobulin (β-Lg) in Balb/c mice used as an animal model of allergy to cow's milk proteins.
Balb/c mice were treated with taurine administered orally by gavage (3mmol/kg/day) or intraperitoneally (100mg/kg/day) for two weeks, then sensitized intraperitoneally with β-Lg. The electrophysiological parameters: active ion transport of chloride (Short-circuit current: Isc) and the passive ion permeability (Conductance: G) were measured ex vivo in Ussing chamber by intestine challenge with β-Lg. Histological study was used to assess gut inflammation. Serum levels of TNF-α and IL-6 were measured. Serum IgG and IgE anti-β-Lg were determined by ELISA.
Compared with sensitized mice, β-Lg challenge of intestinal epithelium of taurine-pre-treated mice in Ussing chamber did not influence the intensity of Isc, nor produce any changes in the G, reflecting a reduction in the secretory response and epithelial permeability. Histological and morphometric analysis showed that taurine reduced the intestinal damage and limited intestine retraction caused by β-Lg sensitization. No statistically significant difference in the serum levels of TNF-α or IL-6 was found after oral or intraperitoneal administration of taurine. Treatment with taurine significantly decreased the IgG (p<0.001) and IgE anti β-Lg levels (p<0.05).
These results have for the first time provided evidence that pre-treatment with taurine appears to prevent intestinal damage induced by β-Lg.
牛奶蛋白过敏常与婴儿慢性炎症引起的肠黏膜功能障碍有关。本研究以对牛奶蛋白过敏的动物模型Balb/c小鼠为对象，评估了牛磺酸对β-乳球蛋白（β-Lg）诱导的肠道损伤的保护作用。
对Balb/c小鼠进行为期两周的牛磺酸灌胃（3mmol/kg/天）或腹腔注射（100mg/kg/天）处理，然后腹腔注射β-Lg使其致敏。通过在Ussing室中用β-Lg刺激肠道，体外测量电生理参数：氯离子的主动离子转运（短路电流：Isc）和被动离子通透性（电导：G）。采用组织学研究评估肠道炎症。检测血清中TNF-α和IL-6的水平。通过酶联免疫吸附测定法（ELISA）测定血清中抗β-Lg的IgG和IgE。
与致敏小鼠相比，在Ussing室中用β-Lg刺激牛磺酸预处理小鼠的肠上皮，并未影响Isc的强度，也未使G发生任何变化，这反映出分泌反应和上皮通透性降低。组织学和形态计量学分析表明，牛磺酸减轻了β-Lg致敏引起的肠道损伤并限制了肠退缩。口服或腹腔注射牛磺酸后，血清中TNF-α或IL-6水平未发现统计学上的显著差异。牛磺酸治疗显著降低了IgG（p<0.001）和抗β-Lg的IgE水平（p<0.05）。
这些结果首次提供了证据，表明牛磺酸预处理似乎可预防β-Lg诱导的肠道损伤。
