Beaudenon S, Praetorius F, Kremsdorf D, Lutzner M, Worsaae N, Pehau-Arnaudet G, Orth G
J Invest Dermatol. 1987 Feb;88(2):130-5. doi: 10.1111/1523-1747.ep12525278.
Lesions from 10 patients suffering from focal epithelial hyperplasia (FEH) of the oral mucosa, including those of 4 Greenlandic Eskimos, were investigated for the presence of human papillomavirus (HPV) DNA sequences by blot hybridization experiments. Two distinct HPVs were detected in the DNA extracted from these lesions, and their genomes were molecularly cloned and characterized. One of these HPVs, detected in 4 patients, was found to be identical with HPV13, whose association with FEH was already known. The other one, detected in 6 patients, was only weakly related to HPV13 and to the other HPVs associated with lesions of the mucous membranes, and constituted a new HPV type, tentatively named HPV32. Lesions from other types of oral papillomas, obtained from 14 additional patients, were also analyzed. Human papillomavirus DNA sequences were detected in the DNA preparations extracted from 5 specimens: HPV6 DNA in a condyloma and in a papilloma, 2 as yet uncharacterized HPV DNAs in 2 papillomas, and HPV32 DNA in a papilloma which showed histologic similarities to FEH. Thus, it seems likely that FEH of the oral mucosa is a disease associated with 2 specific HPVs--HPV13 and HPV32.
通过印迹杂交实验，对10例口腔黏膜局灶性上皮增生（FEH）患者的病变组织进行了研究，其中包括4例格陵兰爱斯基摩人的病变组织，以检测人乳头瘤病毒（HPV）DNA序列。从这些病变组织提取的DNA中检测到两种不同的HPV，并对其基因组进行了分子克隆和特性分析。在4例患者中检测到的其中一种HPV与HPV13相同，其与FEH的关联已为人所知。在6例患者中检测到的另一种HPV与HPV13以及与黏膜病变相关的其他HPV仅有微弱关联，构成了一种新的HPV类型，暂命名为HPV32。还对另外14例患者的其他类型口腔乳头瘤的病变组织进行了分析。在从5个标本提取的DNA制剂中检测到了人乳头瘤病毒DNA序列：在1例尖锐湿疣和1例乳头瘤中检测到HPV6 DNA，在2例乳头瘤中检测到2种尚未鉴定的HPV DNA，在1例组织学上与FEH相似的乳头瘤中检测到HPV32 DNA。因此，口腔黏膜FEH似乎是一种与两种特定HPV（HPV13和HPV32）相关的疾病。
