Hiramatsu K, Nishida J, Naito A, Yoshikura H
J Gen Virol. 1987 Jan;68 ( Pt 1):213-8. doi: 10.1099/0022-1317-68-1-213.
A DNA clone of human T cell leukaemia virus type I (HTLV-I) was isolated from extrachromosomal closed circular copies in chronically infected promyelocytic leukaemia HL60 cells. The new HTLV-I isolate had an intact reading frame in the gag-pol region which could encode protein of 234 amino acids. This open reading frame has not been observed in previous HTLV-I isolates, although similar open reading frames have been reported in the corresponding locations in the related bovine leukaemia virus and HTLV type II. We consider that this open reading frame codes for the virus-encoded protease, on the basis of the homology of the predicted amino acid sequence with those of previously identified retrovirus proteases.
从慢性感染的早幼粒细胞白血病HL60细胞的染色体外闭合环状拷贝中分离出I型人类T细胞白血病病毒（HTLV-I）的一个DNA克隆。新的HTLV-I分离株在gag-pol区域有一个完整的阅读框，可编码234个氨基酸的蛋白质。尽管在相关的牛白血病病毒和II型HTLV的相应位置已报道有类似的开放阅读框，但在先前的HTLV-I分离株中尚未观察到这种开放阅读框。基于预测的氨基酸序列与先前鉴定的逆转录病毒蛋白酶的氨基酸序列的同源性，我们认为这个开放阅读框编码病毒编码的蛋白酶。
