Systems Biology Laboratory, School of Mathematics and Statistics, and Department of Biomedical Engineering, Melbourne School of Engineering, University of Melbourne, Parkville, Victoria 3010, Australia.
Auckland Bioengineering Institute, University of Auckland, New Zealand.
J Theor Biol. 2019 Nov 21;481:10-23. doi: 10.1016/j.jtbi.2018.09.034. Epub 2018 Sep 28.
Membrane transporters contribute to the regulation of the internal environment of cells by translocating substrates across cell membranes. Like all physical systems, the behaviour of membrane transporters is constrained by the laws of thermodynamics. However, many mathematical models of transporters, especially those incorporated into whole-cell models, are not thermodynamically consistent, leading to unrealistic behaviour. In this paper we use a physics-based modelling framework, in which the transfer of energy is explicitly accounted for, to develop thermodynamically consistent models of transporters. We then apply this methodology to model two specific transporters: the cardiac sarcoplasmic/endoplasmic Ca ATPase (SERCA) and the cardiac Na/K ATPase.
膜转运蛋白通过将底物跨膜转运来调节细胞的内部环境。与所有物理系统一样，膜转运蛋白的行为受到热力学定律的限制。然而，许多转运蛋白的数学模型，特别是那些整合到全细胞模型中的模型，在热力学上并不一致，导致不切实际的行为。在本文中，我们使用基于物理的建模框架，其中明确考虑了能量的传递，来开发转运蛋白的热力学一致模型。然后，我们将该方法应用于两种特定转运蛋白的建模：心肌肌浆网/内质网 Ca ATP 酶（SERCA）和心肌 Na/K ATP 酶。
