Knipe D M, Senechek D, Rice S A, Smith J L
J Virol. 1987 Feb;61(2):276-84. doi: 10.1128/JVI.61.2.276-284.1987.
The nuclear localization of the herpes simplex virus transcriptional activator protein ICP4 was studied by indirect immunofluorescence. At early times after viral infection, ICP4 quickly localized to a diffuse intranuclear distribution. ICP4 later concentrated in globular compartments within the nucleus. The redistribution to the compartments was dependent on viral DNA replication. Double staining for ICP4 and ICP8, the early major DNA-binding protein, revealed that both were found in the same intranuclear globular compartments at late times. These were previously named "replication compartments" (M. P. Quinlan, L. B. Chen, and D. M. Knipe, Cell 36:857-868, 1984). Because ICP4 and ICP8 are known to function in transcriptional activation and DNA replication, respectively, both DNA replication and late transcription may occur in these compartments. The association of ICP4 and ICP8 with the replication compartments appeared to be independent in that the retention of ICP8 in the compartments required ongoing viral DNA synthesis, while the association of ICP4 was independent of viral DNA synthesis once the compartments were formed. Because ICP4 shows a different distribution at early and late times, stimulation of transcription by ICP4 may involve different molecular events or contacts during these two periods of the replicative cycle.
通过间接免疫荧光法研究了单纯疱疹病毒转录激活蛋白ICP4的核定位。在病毒感染后的早期，ICP4迅速定位于弥散的核内分布。ICP4随后集中在细胞核内的球状区室中。向这些区室的重新分布依赖于病毒DNA复制。对ICP4和早期主要DNA结合蛋白ICP8进行双重染色，结果显示在后期两者都存在于相同的核内球状区室中。这些区室先前被命名为“复制区室”（M. P. 昆兰、L. B. 陈和D. M. 克尼普，《细胞》36:857 - 868，1984年）。因为已知ICP4和ICP8分别在转录激活和DNA复制中发挥作用，所以DNA复制和晚期转录可能都发生在这些区室中。ICP4和ICP8与复制区室的关联似乎是独立的，因为ICP8在区室中的保留需要持续的病毒DNA合成，而一旦区室形成，ICP4与区室的关联则独立于病毒DNA合成。由于ICP4在早期和晚期表现出不同的分布，所以ICP4在转录激活过程中，在复制周期的这两个阶段可能涉及不同的分子事件或相互作用。
