Xie Yang, Hu Deng, Zhong Cheng, Liu Kai-Fei, Fang En, Zhang Ying-Jun, Zhou Chun, Tian Li-Wen
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Xianning Institute of Drug Supervision and Inspection, Xianning 437100, China.
Steroids. 2018 Dec;140:70-76. doi: 10.1016/j.steroids.2018.09.011. Epub 2018 Sep 29.
Seven new furostanol saponins (1-7), chongrenosides A-G, were isolated from the rhizomes of Smilax china L., together with nine known furostanol saponins (8-16). The structures of the new furostanol saponins (1-7) were elucidated by extensive spectroscopic data analyses (1D and 2D NMR, HRESIMS) and chemical evidence. Compounds 1-6 and 8-16 were evaluated for TNF-α mRNA expression inhibitory activity on LPS induced RAW264.7 cells. Of them, 1, 4, 6, and 11 inhibited the TNF-α mRNA expression by 88%, 87%, 67%, and 93%, respectively, at the concentration of 10 µM.
从菝葜根茎中分离得到7个新的呋甾烷醇皂苷（1-7），即重楼皂苷A-G，以及9个已知的呋甾烷醇皂苷（8-16）。通过广泛的光谱数据分析（一维和二维核磁共振、高分辨电喷雾电离质谱）和化学证据阐明了新呋甾烷醇皂苷（1-7）的结构。对化合物1-6和8-16进行了脂多糖诱导的RAW264.7细胞中肿瘤坏死因子-α mRNA表达抑制活性的评估。其中，在10 μM浓度下，1、4、6和11分别抑制肿瘤坏死因子-α mRNA表达88%、87%、67%和93%。
