McCormick B M, Driesen S J, Connaughton I D, Monckton R P
Res Vet Sci. 1986 Nov;41(3):397-401.
Surveys of serum antibodies to enterovirus serotype 8 and parvovirus were conducted in pigs three to 21 weeks old using the virus neutralisation (VN), enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition tests. Antibody levels to enterovirus were at their lowest at four to eight weeks old, increased at 14 to 16 weeks to high levels which were maintained until 21 weeks. Specific immunoglobulin G (IgG) values measured by ELISA paralleled VN values and were similar in two separate farm surveys. Measurement of immunoglobulin M (IgM) levels indicated that enterovirus infection occurred about four weeks old. Sera obtained from a large number of geographically separated farms, from abattoir-bled animals and from colostrum-deprived piglets raised for 33 weeks from birth in an isolated environment were tested by VN. Results revealed that porcine enterovirus serotype 8 is spread widely throughout northern Victorian piggeries and high antibody levels prevail. In contrast to the enterovirus antibody results, parvovirus antibody levels measured in piglets declined from high levels at three to four weeks to undetectable levels from 13 weeks old.
使用病毒中和试验（VN）、酶联免疫吸附测定（ELISA）和血凝抑制试验，对3至21周龄猪的肠道病毒8型和细小病毒血清抗体进行了调查。肠道病毒抗体水平在4至8周龄时最低，在14至16周龄时升高至高水平，并一直维持到21周龄。通过ELISA测定的特异性免疫球蛋白G（IgG）值与VN值平行，并且在两项独立的农场调查中相似。免疫球蛋白M（IgM）水平的测量表明肠道病毒感染发生在约4周龄时。通过VN对从大量地理上分散的农场、屠宰场采血的动物以及在隔离环境中从出生起饲养33周的初乳剥夺仔猪获得的血清进行了检测。结果显示，猪肠道病毒8型在维多利亚州北部的养猪场中广泛传播，且抗体水平较高。与肠道病毒抗体结果相反，仔猪中测得的细小病毒抗体水平从3至4周龄时的高水平下降至13周龄时的不可检测水平。
