Lukose Jijo, N Mithun, M Priyanka, Mohan Ganesh, Shastry Shamee, Chidangil Santhosh
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education Manipal Karnataka India 576104
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education Manipal Karnataka India 576104.
RSC Adv. 2019 May 21;9(28):15933-15940. doi: 10.1039/c9ra01840a. eCollection 2019 May 20.
The dermal penetration of bisphenol-A (BPA) from thermal papers into the human skin is a matter of major health concern due to its extensive use in developing countries like India, one of its largest users in the world. Bisphenol A is widely used in the manufacture of many consumer goods like polycarbonate water bottles, baby bottles, food containers, home appliances, thermal papers used in billing and tickets, the inner lining of food cans, BPA can be easily adsorbed into the blood rapidly. The integration of optical tweezers with Raman spectroscopic techniques has realized avenues for interpreting single cell investigations. In the present work, the impact of BPA from thermal papers on individual human erythrocytes (red blood cells) has been investigated using micro-Raman spectroscopy. Significant intensity variations were noticed for hemoglobin oxygenation markers in the Raman spectra of red blood cells (RBCs). Raman spectral variations supporting RBC hemoglobin depletion were also found in the presence of BPA. Evident morphological changes are also observed in RBCs due to BPA exposures, which ultimately lead to cell bursting at higher concentrations.
由于双酚A（BPA）在印度等发展中国家被广泛使用，而印度是全球最大的BPA使用国之一，因此热敏纸上的BPA渗透到人体皮肤中成为一个重大的健康问题。双酚A广泛用于制造许多消费品，如聚碳酸酯水瓶、奶瓶、食品容器、家用电器、用于计费和票据的热敏纸、食品罐头的内衬，BPA很容易迅速被吸收到血液中。光镊与拉曼光谱技术的结合为解释单细胞研究开辟了道路。在目前的工作中，使用显微拉曼光谱研究了热敏纸上的BPA对单个人类红细胞的影响。在红细胞（RBC）的拉曼光谱中，血红蛋白氧合标记物出现了显著的强度变化。在存在BPA的情况下，还发现了支持RBC血红蛋白消耗的拉曼光谱变化。由于BPA暴露，红细胞也出现了明显的形态变化，最终在高浓度下导致细胞破裂。
