CellCOM-SB Research Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), University of A Coruña, Servizo Galego de Saúde (SERGAS), Xubias de Arriba, 84 15006 A Coruña, Spain; Epigenetic and Cellular Senescence Group, Blizard Institute, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK.
CellCOM-SB Research Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), University of A Coruña, Servizo Galego de Saúde (SERGAS), Xubias de Arriba, 84 15006 A Coruña, Spain.
Biochim Biophys Acta Biomembr. 2018 Dec;1860(12):2499-2505. doi: 10.1016/j.bbamem.2018.09.009. Epub 2018 Sep 14.
Cell-to-cell communication between bone, cartilage and the synovial membrane is not fully understood and it is only attributed to the diffusion of substances through the extracellular space or synovial fluid. In this study, we found for the first time that primary bone cells (BCs) including osteocytes, synovial cells (SCs) and chondrocytes (CHs) are able to establish cellular contacts and to couple through gap junction (GJ) channels with connexin43 (Cx43) being dominant. Transwell co-culture and identification by mass spectrometry revealed the exchange of essential amino acids, peptides and proteins including calnexin, calreticulin or CD44 antigen between contacting SCs, BCs and CHs. These results reveal that CHs, SCs and BCs are able to establish intercellular connections and to communicate through GJ channels, which provide a selective signalling route by the direct exchange of potent signalling molecules and metabolites.
骨、软骨和滑膜之间的细胞间通讯尚未完全阐明，目前仅归因于物质通过细胞外空间或滑液的扩散。在这项研究中，我们首次发现原代骨细胞（BCs）包括骨细胞、滑膜细胞（SCs）和软骨细胞（CHs）能够建立细胞接触，并通过缝隙连接（GJ）通道与连接蛋白 43（Cx43）相偶联。Transwell 共培养和质谱鉴定揭示了接触的 SCs、BCs 和 CHs 之间必需氨基酸、肽和蛋白质（包括钙连蛋白、钙网蛋白或 CD44 抗原）的交换。这些结果表明 CHs、SCs 和 BCs 能够建立细胞间连接，并通过 GJ 通道进行通讯，这提供了一种通过直接交换有效的信号分子和代谢物的选择性信号转导途径。
