Lee E, Fernon C, Simpson R, Weir R C, Rice C M, Dalgarno L
Biochemistry Department, Faculty of Science, Australian National University, Canberra.
Virus Genes. 1990 Sep;4(3):197-213. doi: 10.1007/BF00265630.
We have determined the nucleotide sequence of the 3'-terminal half of the RNA genome of Murray Valley encephalitis virus (MVE) using seven overlapping cDNA clones; an estimated 80-90 nucleotides at the extreme 3'-end remain to be sequenced. In conjunction with previous sequence data for the 5' half (16), we can conclude that the MVE genome contains a long open reading frame of 10,302 nucleotides that encodes a polyprotein of 3434 residues. Comparison of the MVE deduced amino acid sequence with that of other flaviviruses shows that MVE is most closely related to Japanese encephalitis virus, consistent with serological studies. Using N-terminal amino acid sequence analysis, three nonstructural proteins (NS1, NS3, and NS5) have been identified and mapped on the MVE genome. MVE NS3 contains sequence motifs suggesting that its amino terminus may function as a serine protease. The central region of NS3 (in the linear amino acid sequence) has motifs that are found in NTP-binding proteins and helicases. MVE NS5 contains a conserved Gly-Asp-Asp sequence that is thought to be essential for RNA-dependent RNA polymerases.
我们利用七个重叠的cDNA克隆确定了墨累谷脑炎病毒（MVE）RNA基因组3'-末端一半的核苷酸序列；在最末端的3'-端估计还有80 - 90个核苷酸有待测序。结合先前关于5'-端一半的序列数据（16），我们可以得出结论，MVE基因组包含一个10302个核苷酸的长开放阅读框，编码一个由3434个残基组成的多蛋白。将MVE推导的氨基酸序列与其他黄病毒的序列进行比较表明，MVE与日本脑炎病毒关系最为密切，这与血清学研究结果一致。通过N端氨基酸序列分析，已鉴定出三种非结构蛋白（NS1、NS3和NS5）并将其定位在MVE基因组上。MVE NS3包含的序列基序表明其氨基末端可能作为丝氨酸蛋白酶发挥作用。NS3的中央区域（在线性氨基酸序列中）具有在NTP结合蛋白和解旋酶中发现的基序。MVE NS5包含一个保守的甘氨酸 - 天冬氨酸 - 天冬氨酸序列，该序列被认为对RNA依赖性RNA聚合酶至关重要。
