用限制性内切酶Eco RI切割腺病毒DNA的动力学研究。
Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.
作者信息
Forsblom S, Rigler R, Ehrenberg M, Philipson L
出版信息
Nucleic Acids Res. 1976 Dec;3(12):3255-69. doi: 10.1093/nar/3.12.3255.
Abstract
The kinetics of cleavage of DNA from Adenovirus Type 1 (Ad1), Type 5 (Ad5) and Type 6 (Ad6) by restriction endonuclease EcoRI was investigated by quantitative evaluation of the fluorescence from ethidium stained DNA fragments separated on agarose gels. The apparent rate constants of cleavage at different cleavage sites have been determined and large differences in the cleavage rates of the individual sites within one type of DNA were found. From the kinetics of cleavage information on the sequence of the DNA fragments can be obtained. The order of the fragment A, B, C, D of Ad6 DNA obtained after complete cleavage by restriction endonuclease Eco RI was found to be A-D-C-B; the order of the corresponding fragments A, B, C of Ad1 and Ad5 DNA was found to be A-C-B.
摘要
通过对琼脂糖凝胶上分离的经溴化乙锭染色的DNA片段荧光进行定量评估，研究了限制性内切酶EcoRI对1型腺病毒（Ad1）、5型腺病毒（Ad5）和6型腺病毒（Ad6）DNA的切割动力学。已确定了不同切割位点的表观速率常数，并发现同一类型DNA内各个位点的切割速率存在很大差异。从切割动力学可以获得有关DNA片段序列的信息。发现经限制性内切酶Eco RI完全切割后得到的Ad6 DNA片段A、B、C、D的顺序为A-D-C-B；Ad1和Ad5 DNA相应片段A、B、C的顺序为A-C-B。
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