Wang Lude, Chen Yang
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, People's Republic of China.
Mikrochim Acta. 2018 Oct 2;185(10):489. doi: 10.1007/s00604-018-3027-8.
A colorimetric and fluorescent pH probe was designed by doping carbon dots (C-dots) with Eu(III), Tb(III) and 2,6-pyridinedicarboxylic acid (DPA). The resulting nanoparticles were applied as fluorescent indicators for pH values (best detected at excitation/emission wavelengths of 272/545, 614 nm). The pH induced optical effects are due to pH induced variations in energy transfer. The fluorescence of the probe shows a continuous color variation, and a linear change with pH values in the range from 3.0 to 10.0 can be established by using a Commission Internationale de L'Eclairage (CIE) chromaticity diagram. This new kind of pH nanoprobe is more accurate than previously reported pH indicator probes because the pH value can be calculated by using chromaticity coordinates that only depend on the chromaticity. The pH nanoprobe was applied to visualize pH values in human breast adenocarcinoma cells (MCF-7). Graphical abstract Carbon dots modified with Eu(III) and Tb(III) complexes of 2,6-pyridinedicarboxylic acid (DPA) were prepared. The doped carbon dots were used as a pH-sensitive nanosensor. The fluorescence chromaticity of the nanoparticles changes with the variation of pH value.
通过用铕（III）、铽（III）和2,6 - 吡啶二甲酸（DPA）掺杂碳点（C - 点）设计了一种比色和荧光pH探针。所得纳米颗粒用作pH值的荧光指示剂（在激发/发射波长为272/545、614 nm时检测效果最佳）。pH诱导的光学效应归因于能量转移中pH诱导的变化。该探针的荧光呈现连续的颜色变化，并且通过使用国际照明委员会（CIE）色度图，可以建立在3.0至10.0范围内与pH值的线性变化关系。这种新型pH纳米探针比先前报道的pH指示剂探针更准确，因为pH值可以通过仅依赖于色度的色度坐标来计算。该pH纳米探针用于可视化人乳腺腺癌细胞（MCF - 7）中的pH值。图形摘要 制备了用2,6 - 吡啶二甲酸（DPA）的铕（III）和铽（III）配合物修饰的碳点。掺杂的碳点用作pH敏感纳米传感器。纳米颗粒的荧光色度随pH值的变化而变化。
