WestCHEM, Department of Pure and Applied Chemistry , University of Strathclyde , Technology and Innovation Centre, 99 George Street , Glasgow , G1 1RD , U.K.
Anal Chem. 2018 Nov 6;90(21):12944-12950. doi: 10.1021/acs.analchem.8b03572. Epub 2018 Oct 16.
The expansion of electrochemical sensors to biomedical applications at point of care requires these sensors to undergo analysis without any pretreatment or extraction. This poses a major challenge for all electrochemical sensors including electrochemiluminescent (ECL)-based sensors. ECL offers many advantages for biomedical applications; however, obtaining results from complex matrixes has proven to be a large hurdle for the application of ECL sensors within this field. This work demonstrates the potential of cathodic ECL to detect and quantify homocysteine (Hcy) with a 0.1 nM limit of detection, which is associated with hyperhomocysteinemia, in blood. This near-infrared quantum dot (NIR QD)-based ECL sensor displays good linearity allowing for rapid detection and providing a basis for exploitation of ECL-based sensors for biomedical diagnostics utilizing Hcy as a model cathodic coreactant. This work will lay the foundations for future developments in biosensing and imaging fields and stands as an initial proof of concept for the utilization of cathodic ECL technologies for biomedical applications once the limits of detection within clinically relevant levels has been achieved. This work illustrates the potential of cathodic ECL sensors, using Hcy as a model complex, for the detection of biomolecules.
电化学传感器在即时检测（point of care）中的生物医学应用需要这些传感器在无需任何预处理或提取的情况下进行分析。这对所有电化学传感器（包括基于电致化学发光（ECL）的传感器）都构成了重大挑战。ECL 为生物医学应用提供了许多优势；然而，从复杂基质中获得结果已被证明是在该领域应用 ECL 传感器的一个巨大障碍。本工作展示了阴极 ECL 检测和定量同型半胱氨酸（Hcy）的潜力，其检测限低至 0.1 nM，与高同型半胱氨酸血症有关，可用于血液检测。这种基于近红外量子点（NIR QD）的 ECL 传感器具有良好的线性度，允许快速检测，并为利用 Hcy 作为模型阴极共反应物的基于 ECL 的生物医学诊断传感器的开发提供了基础。本工作将为生物传感和成像领域的未来发展奠定基础，并为一旦达到临床相关水平的检测限，就阴极 ECL 技术在生物医学应用中的利用提供初步的概念验证。本工作说明了以 Hcy 为模型复杂分子的阴极 ECL 传感器在生物分子检测方面的潜力。
