LncRNA LET 在乳腺癌发展中作为肿瘤抑制因子发挥作用。
LncRNA LET function as a tumor suppressor in breast cancer development.
机构信息
Department of Thyroid Breast Surgery, Liaocheng People's Hospital, Liaocheng, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Sep;22(18):6002-6007. doi: 10.26355/eurrev_201809_15935.
OBJECTIVE
To evaluate the effect of long-chain non-coding RNA LET (lncRNA LET) on the regulatory of human breast cancer and its underlying mechanism.
PATIENTS AND METHODS
The expression levels of lncRNA LET in breast cancer tissues, MDA-MB-231 cells and MCF-10A breast epithelial cells were detected by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The proliferation of lncRNA LET was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). Cell apoptosis was examined via flow cytometry. The invasion and migration of cells were detected by transwell and scratch assay.
RESULTS
The expression of lncRNA LET was reduced in breast cancer tissues and MDA-MB-231 cells. Overexpression of lncRNA LET resulted in the inhibition of cell proliferation, invasion and migration ability, and promotion of cell apoptosis (p<0.05). Up-regulation of lncRNA LET repressed epithelial mesenchymal transition (EMT) process.
CONCLUSIONS
LncRNA LET is a new type of molecule involved in the development of breast cancer, which may become a potential target for the treatment of breast cancer.
目的
评估长链非编码 RNA LET（lncRNA LET）对人乳腺癌的调控作用及其潜在机制。
患者与方法
采用实时定量聚合酶链反应（qRT-PCR）检测乳腺癌组织、MDA-MB-231 细胞和 MCF-10A 乳腺上皮细胞中 lncRNA LET 的表达水平。MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）检测 lncRNA LET 的增殖情况。通过流式细胞术检测细胞凋亡。通过 Transwell 和划痕实验检测细胞的侵袭和迁移能力。
结果
lncRNA LET 在乳腺癌组织和 MDA-MB-231 细胞中表达下调。过表达 lncRNA LET 导致细胞增殖、侵袭和迁移能力受到抑制，细胞凋亡增加（p<0.05）。lncRNA LET 的上调抑制了上皮间质转化（EMT）过程。
结论
lncRNA LET 是一种参与乳腺癌发生发展的新型分子，可能成为治疗乳腺癌的潜在靶点。
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