Onder Sakip, Calikoglu-Koyuncu Ayse Ceren, Kazmanli Kursat, Urgen Mustafa, Kok Fatma Nese, Torun-Kose Gamze
Biomedical Engineering Department, Yıldız Technical University, Istanbul, Turkey.
Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey.
Biomed Mater Eng. 2018;29(4):427-438. doi: 10.3233/BME-181000.
In vitro evaluation of cell-surface interactions for hard tissue implants have mostly been done using osteoblasts. However, when an implant is placed in the body, mesenchymal stem cells (MSCs) play a major role in new bone formation. Therefore, using MSCs in cell-surface investigations may provide more reliable information on the prediction of in vivo behavior of implants.
In this study, Mg doped TiN coatings ((Ti,Mg)N) were prepared and tested for their effect on MSC differentiation and mineralization.
MSCs were isolated from rat bone marrow (rBMSCs) and seeded onto bare Ti, TiN and Mg containing (Ti,Mg)N surfaces. Cell proliferation, osteogenic differentiation (collagen type 1, alkaline phosphatase activity), calcium phosphate deposition (von Kossa staining, Scanning Electron Microscopy) analysis were conducted.
Differentiation towards osteoblast lineage was significantly improved with the increment in Mg presence. Collagen type I deposition, mineralization, and the ALP activity were higher on high Mg containing (>10 at% Mg) surfaces but differentiation of rBMSCs were found to be delayed.
Mg presence affected rBMSCs proliferation and differentiation positively in a dose-dependent manner. However, high Mg amounts delayed both proliferation and differentiation.
对于硬组织植入物，细胞表面相互作用的体外评估大多使用成骨细胞进行。然而，当植入物植入体内时，间充质干细胞（MSC）在新骨形成中起主要作用。因此，在细胞表面研究中使用MSC可能为预测植入物的体内行为提供更可靠的信息。
本研究制备了掺镁TiN涂层（(Ti,Mg)N），并测试其对MSC分化和矿化的影响。
从大鼠骨髓中分离出MSC（rBMSC），接种到裸露的钛、TiN和含镁（(Ti,Mg)N）表面。进行细胞增殖、成骨分化（I型胶原蛋白、碱性磷酸酶活性）、磷酸钙沉积（冯科萨染色、扫描电子显微镜）分析。
随着镁含量的增加，向成骨细胞谱系的分化显著改善。在高镁含量（>10原子%镁）表面，I型胶原蛋白沉积、矿化和碱性磷酸酶活性更高，但发现rBMSC的分化延迟。
镁的存在以剂量依赖的方式对rBMSC的增殖和分化产生积极影响。然而，高镁含量会延迟增殖和分化。
