Wrocław University of Environmental and Life Sciences, Department Of Biotechnology and Food Microbiology, Chełmońskiego 37, 51-630, Wrocław, Poland.
Newcastle University, Institute for Cell and Molecular Biosciences, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom.
Sci Rep. 2018 Oct 3;8(1):14735. doi: 10.1038/s41598-018-33168-6.
Erythritol production is a unique response to hyperosmotic stress that is observed in a small group of yeasts, including Yarrowia lipolytica. This study investigated whether this unusual mechanism is regulated by the HOG pathway, well described in Saccharomyces cerevisiae. The gene YALI0E25135g was identified as the Y. lipolytica homologue of HOG1 and was found to be phosphorylated in response to hyperosmotic shock. Deletion of the gene caused a significant decrease in resistance to hyperosmotic stress and negatively affected erythritol production. Interestingly, the deletion strain yl-hog1Δ displayed significant morphological defects, with the cells growing in a filamentous form. Moreover, yl-hog1Δ cells were also resistant to the cell wall damaging agents Congo red and calcofluor white. Collectively, these results indicate that yl-Hog1 is crucial for the cellular response to hyperosmotic stress, plays a role in the induction of erythritol production, and potentially prevents cross-talk with different MAPK signalling pathways in the cell.
赤藓糖醇的生产是一种对高渗胁迫的独特反应，这种反应仅在少数酵母中观察到，包括解脂耶氏酵母。本研究调查了这种不寻常的机制是否受 Hog 途径调控，而 Hog 途径在酿酒酵母中已有详细描述。鉴定出 YALI0E25135g 基因为 Hog1 的解脂耶氏酵母同源基因，并且该基因在响应高渗冲击时发生磷酸化。该基因的缺失导致对高渗胁迫的抗性显著降低，并对赤藓糖醇的生产产生负面影响。有趣的是，hog1Δ 缺失株表现出明显的形态缺陷，细胞呈丝状生长。此外，yl-hog1Δ 细胞对细胞壁损伤剂刚果红和钙荧光白也有抗性。总之，这些结果表明 yl-Hog1 对细胞响应高渗胁迫至关重要，在诱导赤藓糖醇生产中发挥作用，并可能防止细胞内不同 MAPK 信号通路之间的串扰。
