Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, Wrocław, 57-630, Poland.
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, Paris, France.
Sci Rep. 2017 Oct 2;7(1):12507. doi: 10.1038/s41598-017-12715-7.
The gene YALI0F01562g was identified as an important factor involved in erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative analysis of four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. The presence of a mutation that seriously damaged the gene corresponded to inability of the strain Wratislavia K1 to utilize erythritol. RT-PCR analysis of the strain MK1 demonstrated a significant increase in YALI0F01562g expression during growth on erythritol. Further studies involving deletion and overexpression of the selected gene showed that it is indeed essential for efficient erythritol assimilation. The deletion strain Y. lipolytica AMM∆euf1 was almost unable to grow on erythritol as the sole carbon source. When the strain was applied in the process of erythritol production from glycerol, the amount of erythritol remained constant after reaching the maximal concentration. Analysis of the YALI0F01562g gene sequence revealed the presence of domains characteristic for transcription factors. Therefore we suggest naming the studied gene Erythritol Utilization Factor - EUF1.
基因 YALI0F01562g 被鉴定为非传统酵母解脂耶氏酵母（Yarrowia lipolytica）中赤藓糖醇分解代谢的重要因素。通过对 4 株解脂耶氏酵母（A-101.1.31、Wratislavia K1、MK1 和 AMM）的比较分析，首次确定了其假定作用。该基因的一个突变严重损伤，这与菌株 Wratislavia K1 无法利用赤藓糖醇相对应。对菌株 MK1 的 RT-PCR 分析表明，在赤藓糖醇上生长时，YALI0F01562g 的表达显著增加。对选定基因进行缺失和过表达的进一步研究表明，该基因对于高效赤藓糖醇同化确实是必需的。缺失菌株 Y. lipolytica AMM∆euf1 几乎无法以赤藓糖醇作为唯一碳源生长。当该菌株应用于从甘油生产赤藓糖醇的过程中，当达到最大浓度后，赤藓糖醇的量保持不变。对 YALI0F01562g 基因序列的分析表明，存在转录因子特征的结构域。因此，我们建议将研究的基因命名为赤藓糖醇利用因子 - EUF1。
