Dobay Orsolya, Laub Krisztina, Stercz Balázs, Kéri Adrienn, Balázs Bernadett, Tóthpál Adrienn, Kardos Szilvia, Jaikumpun Pongsiri, Ruksakiet Kasidid, Quinton Paul M, Zsembery Ákos
Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary.
Department of Oral Biology, Semmelweis University, Budapest, Hungary.
Front Microbiol. 2018 Sep 19;9:2245. doi: 10.3389/fmicb.2018.02245. eCollection 2018.
We investigated the effects of bicarbonate on the growth of several different bacteria as well as its effects on biofilm formation and intracellular cAMP concentration in . Biofilm formation was examined in 96-well plates, with or without bicarbonate. The cAMP production of bacteria was measured by a commercial assay kit. We found that NaHCO (100 mmol l) significantly inhibited, whereas NaCl (100 mmol l) did not influence the growth of planktonic bacteria. MIC and MBC measurements indicated that the effect of is bacteriostatic rather than bactericidal. Moreover, NaHCO prevented biofilm formation as a function of concentration. Bicarbonate and alkalinization of external pH induced a significant increase in intracellular cAMP levels. In conclusion, impedes the planktonic growth of different bacteria and impedes biofilm formation by that is associated with increased intracellular cAMP production. These findings suggest that aerosol inhalation therapy with solutions may help improve respiratory hygiene in patients with cystic fibrosis and possibly other chronically infected lung diseases.
我们研究了碳酸氢盐对几种不同细菌生长的影响，以及它对生物膜形成和细胞内cAMP浓度的影响。在有无碳酸氢盐的情况下，于96孔板中检测生物膜形成。细菌的cAMP产生通过商用检测试剂盒进行测量。我们发现，NaHCO₃（100 mmol/L）显著抑制浮游细菌的生长，而NaCl（100 mmol/L）对其生长没有影响。最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测量表明，其作用是抑菌而非杀菌。此外，NaHCO₃可根据浓度阻止生物膜形成。碳酸氢盐和外部pH的碱化导致细胞内cAMP水平显著升高。总之，碳酸氢盐阻碍不同细菌的浮游生长，并通过与细胞内cAMP产生增加相关的机制阻碍生物膜形成。这些发现表明，用碳酸氢盐溶液进行雾化吸入治疗可能有助于改善囊性纤维化患者以及可能其他慢性感染性肺部疾病患者的呼吸道卫生状况。
