Muhammed Ali Ruaa Ali, Ridha Alshara Jamal Mohammed, Saaid Tuwaij Nabil Salim, Baker Al-Khilkhali Huda Jameel
The Gifted Students' School in Najaf, Ministry of Education, Iraq.
Faculty of Dentistry, University of Kufa, Kufa, Iraq.
Rep Biochem Mol Biol. 2022 Apr;11(1):166-175. doi: 10.52547/rbmb.11.1.166.
remains one of the leading agents of urinary tract infection (UTIs), it has become resistant to many drugs. Current work aimed to evaluate some chemical substances as antibacterial agents and molecular study of virulence factors associated with UTIs.
This work involved 133 urine specimens obtained from females' patients suffering from UTIs, Methods of well diffusion and disk diffusion were achieved to assay the effect of some chemical substances and antibiogram profiles toward Sulfamethoxazole-trimethoprim (SXT)-resistant respectively. Virulence genes were done based on the technique of Polymerase Chain Reaction (PCR).
The results recorded 49/133 (36.84%) among women suffering UTIs, 28/49 (57.14%) were resistant to SXT drug. imipenem, meropenem, and nitrofurantoin were recorded more effectively. Chemicals substances at the concentration 0.3 (g/ml) recorded percentages of inhibition, reaching 9.143±1.442, 15.36±0.5914, and 21.82±0.8699 for NaHCO3, Ch4c, and Viroxide Super™ respectively. PCR demonstrated that 28/28 (100%) of SXT-resistant isolates were harbored and genes, while 14/28 (50%), 15/28 (53.57%), 19/28 (67.85%) and 26/28 (92.85%) in and genes, respectively. gene was not observed.
Observed a high percentage of that were resistant to SXT drug, and having several virulence genes, poses a real threat, it requires a real pause to create substitutions to limit the spreading of this threat.
仍是尿路感染（UTIs）的主要病原体之一，它已对多种药物产生耐药性。当前的工作旨在评估一些化学物质作为抗菌剂以及与UTIs相关的毒力因子的分子研究。
这项工作涉及从患有UTIs的女性患者中获取的133份尿液标本，采用平板打孔法和纸片扩散法分别测定一些化学物质和抗菌谱对磺胺甲恶唑-甲氧苄啶（SXT）耐药菌的作用。基于聚合酶链反应（PCR）技术检测毒力基因。
结果显示，在患有UTIs的女性中，49/133（36.84%）检测到该菌，其中28/49（57.14%）对SXT药物耐药。亚胺培南、美罗培南和呋喃妥因的效果更佳。浓度为0.3（g/ml）的化学物质NaHCO3、Ch4c和Viroxide Super™的抑菌率分别达到9.143±1.442、15.36±0.5914和21.82±0.8699。PCR结果表明，28/28（100%）的SXT耐药菌株携带某基因，而在另外某些基因中，携带率分别为14/28（50%）、15/28（53.57%）、19/28（67.85%）和26/28（92.85%）。未观察到某基因。
观察到该菌对SXT药物有较高耐药率且具有多个毒力基因，这构成了真正的威胁，需要切实采取措施寻找替代方法以限制这种威胁的传播。
