Ollivierre F, Gubler U, Towle C A, Laurencin C, Treadwell B V
Biochem Biophys Res Commun. 1986 Dec 30;141(3):904-11. doi: 10.1016/s0006-291x(86)80128-0.
In this report we describe the presence of interleukin-1 activity in medium conditioned by bovine articular cartilage. Preparations partially purified by Sephacryl S200 chromatography (Mr 18000-25000) stimulate murine thymocyte proliferation in the lymphocyte activation factor assay. Furthermore, the factor(s) activate cartilage tissue to secrete a protease which is essential for the activity of purified synovial collagenase. We also demonstrate the presence of mRNA coding for IL-1 alpha and beta in human articular chondrocytes and conclude that the human monocytic and chondrocytic mRNAs are identical. Our results demonstrating cartilage expression of IL-1 genes suggest the possibility of an autocrine mechanism whereby chondrocyte production of matrix degrading proteases is initiated by chondrocyte derived IL-1.
在本报告中，我们描述了牛关节软骨条件培养基中白细胞介素-1活性的存在。通过Sephacryl S200色谱法部分纯化的制剂（分子量18000 - 25000）在淋巴细胞激活因子测定中刺激小鼠胸腺细胞增殖。此外，该因子激活软骨组织分泌一种蛋白酶，这种蛋白酶对于纯化的滑膜胶原酶的活性至关重要。我们还证明了人类关节软骨细胞中编码IL-1α和β的mRNA的存在，并得出结论，人类单核细胞和软骨细胞的mRNA是相同的。我们关于软骨中IL-1基因表达的结果提示了一种自分泌机制的可能性，即软骨细胞衍生的IL-1启动软骨细胞产生基质降解蛋白酶。
