Coatney Garrett A, Abraham Adam C, Fischenich Kristine M, Button Keith D, Haut Roger C, Haut Donahue Tammy L
Soft Tissue Mechanics Laboratory, Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
Orthopaedic Biomechanics Laboratories, College of Osteopathic Medicine, Michigan State University, East Lansing, MI USA.
J Mech Behav Biomed Mater. 2015 Jul;47:57-64. doi: 10.1016/j.jmbbm.2015.03.008. Epub 2015 Mar 21.
Traumatic injury to the knee leads to the development of post-traumatic osteoarthritis. The objective of this study was to characterize the effects of a single intra-articular injection of a non-ionic surfactant, Poloxamer 188 (P188), in preservation of meniscal tissue following trauma through maintenance of meniscal glycosaminoglycan (GAG) content and mechanical properties. Flemish Giant rabbits were subjected to a closed knee joint, traumatic compressive impact with the joint constrained to prevent anterior tibial translation. The contralateral limb served as an un-impacted control. Six animals (treated) received an injection of P188 in phosphate buffered saline (PBS) post trauma, and another six animals (sham) received a single injection of PBS to the impacted limb. Histological analyses for GAG was determined 6 weeks post trauma, and functional outcomes were assessed using stress relaxation micro-indentation. The impacted limbs of the sham group demonstrated a significant decrease in meniscal GAG coverage compared to non-impacted limbs (p<0.05). GAG coverage of the impacted P188 treated limbs was not significantly different than contralateral non-impacted limbs in all regions except the medial anterior (p<0.05). No significant changes were documented in mechanics for either the sham or treated groups compared to their respective control limbs. This suggests that a single intra-articular injection of P188 shows promise in prevention of trauma induced GAG loss.
膝关节创伤会导致创伤后骨关节炎的发生。本研究的目的是通过维持半月板糖胺聚糖（GAG）含量和力学性能，来表征单次关节内注射非离子表面活性剂泊洛沙姆188（P188）对创伤后半月板组织保存的影响。对弗拉芒巨兔进行闭合性膝关节创伤性压缩冲击，同时限制关节以防止胫骨前移。对侧肢体作为未受冲击的对照。六只动物（治疗组）在创伤后接受了P188磷酸盐缓冲盐水（PBS）注射，另外六只动物（假手术组）对受冲击肢体注射了单次PBS。创伤后6周进行GAG的组织学分析，并使用应力松弛微压痕评估功能结果。与未受冲击的肢体相比，假手术组受冲击肢体的半月板GAG覆盖率显著降低（p<0.05）。除内侧前部外，在所有区域中，接受P188治疗的受冲击肢体的GAG覆盖率与对侧未受冲击肢体无显著差异（p<0.05）。与各自的对照肢体相比，假手术组或治疗组的力学性能均未记录到显著变化。这表明单次关节内注射P188在预防创伤诱导的GAG丢失方面显示出前景。
