质粒NAH7的nah和sal操纵子的调控：nahR新功能的证据

Regulation of the nah and sal operons of plasmid NAH7: evidence for a new function in nahR.

作者信息

You I S, Gunsalus I C

出版信息

Biochem Biophys Res Commun. 1986 Dec 30;141(3):986-92. doi: 10.1016/s0006-291x(86)80141-3.

Abstract

The catabolism of naphthalene and salicylate is specified by two operons on an 80 Kb metabolic plasmid, NAH7. These operons, nah and sal, are carried on the contiguous 30 Kb EcoRI-A, C fragments, and are under positive control of a regulator region, nahR. Five Nah Sal Tn5 insertion mutants form two complementation groups: A = nahR203, nahR204; and B = nahR201, nahR202, nahR205. The physical and genetic maps assign the nahR location to the 15.7-17.2 Kb region of the EcoRI-A fragment, with suggestion of more than one control gene.

摘要

萘和水杨酸盐的分解代谢由一个80 Kb的代谢质粒NAH7上的两个操纵子决定。这些操纵子，即nah和sal，位于相邻的30 Kb EcoRI-A、C片段上，并受一个调控区nahR的正调控。五个Nah Sal Tn5插入突变体形成两个互补组：A组 = nahR203、nahR204；B组 = nahR201、nahR202、nahR205。物理图谱和遗传图谱将nahR的位置定位到EcoRI-A片段的15.7 - 17.2 Kb区域，提示存在不止一个控制基因。