State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China.
Adv Mater. 2018 Nov;30(46):e1804023. doi: 10.1002/adma.201804023. Epub 2018 Oct 4.
Pathogenic bacterial infections and drug resistance make it urgent to develop new antibacterial agents with targeted delivery. Here, a new targeting delivery nanosystem is designed based on the potential interaction between bacterial recognizing receptors on macrophage membranes and distinct pathogen-associated molecular patterns in bacteria. Interestingly, the expression of recognizing receptors on macrophage membranes increases significantly when cultured with specific bacteria. Therefore, by coating pretreated macrophage membrane onto the surface of a gold-silver nanocage (GSNC), the nanosystem targets bacteria more efficiently. Previously, it has been shown that GSNC alone can serve as an effective antibacterial agent owing to its photothermal effect under near-infrared (NIR) laser irradiation. Furthermore, the nanocage can be utilized as a delivery vehicle for antibacterial drugs since the gold-silver nanocage presents a hollow interior and porous wall structure. With significantly improved bacterial adherence, the Sa-M-GSNC nanosystem, developed within this study, is effectively delivered and retained at the infection site both via local or systemic injections; the system also shows greatly prolonged blood circulation time and excellent biocompatibility. The present work described here is the first to utilize bacterial pretreated macrophage membrane receptors in a nanosystem to achieve specific bacterial-targeted delivery, and provides inspiration for future therapy based on this concept.
致病细菌感染和耐药性的出现使得靶向递药的新型抗菌剂的开发迫在眉睫。在此，我们设计了一种基于巨噬细胞膜上细菌识别受体与细菌中特定病原体相关分子模式之间潜在相互作用的新型靶向递药纳米系统。有趣的是，巨噬细胞在与特定细菌共培养时，其细胞膜上的识别受体表达显著增加。因此，通过将预处理后的巨噬细胞膜涂覆在金银纳米笼（GSNC）表面，该纳米系统能够更有效地靶向细菌。此前已经表明，由于在近红外（NIR）激光照射下的光热效应，单独的 GSNC 本身可以作为一种有效的抗菌剂。此外，由于金-银纳米笼具有中空内部和多孔壁结构，因此它也可以用作抗菌药物的递送载体。Sa-M-GSNC 纳米系统具有显著提高的细菌黏附性，通过局部或全身注射，能够有效地递送到感染部位并保留在感染部位；该系统还表现出大大延长的血液循环时间和良好的生物相容性。本研究首次利用细菌预处理的巨噬细胞膜受体在纳米系统中实现了特异性细菌靶向递药，为今后基于这一概念的治疗提供了启示。
