Payment P, Fortin S, Trudel M
Can J Microbiol. 1986 Dec;32(12):922-5. doi: 10.1139/m86-170.
The present study was undertaken to determine if viruses were selectively eliminated during waste water treatment. Human enteric viruses were detected at all steps of treatment in a conventional activated sludge waste water treatment plant. Liquid overlays and large volume sampling with multiple passages on BGM cells permitted the detection of poliovirus (serotypes 1, 2, and 3), coxsackievirus B (serotypes 1, 2, 3, 4, and 5), and echovirus (serotypes 3, 14, and 22), as well as reoviruses. The mean virus concentration was 95.1 most probable number of infectious units per litre (mpniu/L) in raw sewage, 23.3 in settled water, 1.4 in effluent after activated sludge treatment, and 40.3 mpniu/L in sludge samples. All samples of raw sewage and settled water, 79% of effluent water, and 94% of sludge samples contained viruses. The mean reduction was 75% after settling and 98% after activated sludge treatment. Poliovirus type 3 was rarely isolated after the activated sludge treatment, but was still detected in about one-third of the sludge samples. Reoviruses and coxsackieviruses were detected at similar rates from all samples and appear to be more resistant to the activated sludge treatment than poliovirus type 3. Poliovirus types 1 and 2 were present in almost every sample of raw sewage and settled water and still found in about half of the effluent and sludge samples, indicating a level of resistance similar to that of reoviruses and coxsackieviruses.
本研究旨在确定病毒在废水处理过程中是否被选择性去除。在一座传统活性污泥法污水处理厂的各个处理步骤中均检测到了人类肠道病毒。采用液体覆盖法和大容量采样，并在BGM细胞上多次传代，从而检测到了脊髓灰质炎病毒（1型、2型和3型）、柯萨奇B组病毒（1型、2型、3型、4型和5型）、埃可病毒（3型、14型和22型）以及呼肠孤病毒。原污水中病毒的平均浓度为每升95.1个最可能感染单位（mpniu/L），沉淀水中为23.3个，活性污泥处理后的出水为1.4个，污泥样本中为40.3 mpniu/L。所有原污水和沉淀水样本、79%的出水样本以及94%的污泥样本都含有病毒。沉淀后平均去除率为75%，活性污泥处理后为98%。活性污泥处理后很少分离到3型脊髓灰质炎病毒，但仍在约三分之一的污泥样本中检测到。从所有样本中检测到呼肠孤病毒和柯萨奇病毒的比例相似，并且它们似乎比3型脊髓灰质炎病毒对活性污泥处理更具抗性。1型和2型脊髓灰质炎病毒几乎存在于每一个原污水和沉淀水样本中，并且仍在约一半的出水和污泥样本中被发现，这表明其抗性水平与呼肠孤病毒和柯萨奇病毒相似。
