Payment P, Ayache R, Trudel M
Can J Microbiol. 1983 Jan;29(1):111-9. doi: 10.1139/m83-018.
In this second study (1979-1981) of the viral content of sewage we have demonstrated the presence of poliovirus types 1, 2, and 3 in Laval and Montreal. Several strains of poliovirus types 2 and 3 were nonvaccinal. This is in contrast with our first study (1977-1978) in which only type 1 poliovirus isolates were nonvaccinal. Coxsackievirus types B-3, B-4, and B-5 and echovirus types 1, 7, and 11 were also isolated from sewage. Interestingly, these isolations coincided with reports of isolation of the same strains during the same period by diagnostic laboratories. Our method based on Vero and BSC-1 cell cultures for virus isolation and immune electron microscopy for identification permitted the recovery not only of several strains of enteroviruses but also of some adenoviruses and reoviruses.
在第二项关于污水病毒含量的研究（1979 - 1981年）中，我们已证实拉瓦尔和蒙特利尔存在1型、2型和3型脊髓灰质炎病毒。2型和3型脊髓灰质炎病毒的几个毒株是非疫苗株。这与我们的第一项研究（1977 - 1978年）形成对比，在第一项研究中只有1型脊髓灰质炎病毒分离株是非疫苗株。B - 3型、B - 4型和B - 5型柯萨奇病毒以及1型、7型和11型埃可病毒也从污水中分离出来。有趣的是，这些分离结果与诊断实验室在同一时期分离出相同毒株的报告相吻合。我们基于Vero细胞和BSC - 1细胞培养进行病毒分离以及利用免疫电子显微镜进行鉴定的方法，不仅能够分离出几种肠道病毒毒株，还能分离出一些腺病毒和呼肠孤病毒。
