Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobelstraße 12, 70569, Stuttgart, Germany.
Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, 72076, Tübingen, Germany.
Macromol Biosci. 2018 Dec;18(12):e1800168. doi: 10.1002/mabi.201800168. Epub 2018 Oct 4.
Gelatin methacryloyl (acetyl) (GM(A)) is increasingly investigated for various applications in life sciences and medicine, for example, drug release or tissue engineering. Gelatin type A and type B are utilized for G M(A) and G M(A) preparation, but the impact of gelatin raw material on modification reaction and resulting polymer properties is rather unknown so far. Therefore, the degrees of modification (DMA) and physicochemical properties of five G M(A) and G M(A) derivatives are compared: The degrees of methacryloylation (0.32-0.98 mmol g ) are indistinguishable for G M(A) and G M(A) as are the sol-gel temperatures. Isoelectric points, solution viscosities, and hydrodynamic radii which are distinct for G and G converge with increasing DMA. Interestingly, differences are measured for the storage moduli and equilibrium degrees of swelling of respective G and G derivative-based hydrogels, in spite of their comparable DMA. This underlines the importance of GM(A) characterization beyond the modification degree.
明胶甲基丙烯酰基（乙酰基）（GM(A)）越来越多地被应用于生命科学和医学的各个领域，例如药物释放或组织工程。明胶 A 型和 B 型被用于 GM(A)和 GM(A)的制备，但到目前为止，明胶原料对修饰反应和所得聚合物性质的影响还知之甚少。因此，比较了五种 GM(A)和 GM(A)衍生物的修饰度（DMA）和物理化学性质：GM(A)和 GM(A)的甲基丙烯酰化程度（0.32-0.98mmol/g）相同，溶胶-凝胶温度也相同。对于 G 和 G，等电点、溶液粘度和流体力学半径是不同的，但随着 DMA 的增加而趋于一致。有趣的是，尽管 DMA 相当，但各自基于 G 和 G 衍生物的水凝胶的储能模量和平衡溶胀度存在差异。这强调了 GM(A)特性表征的重要性，不仅仅是修饰程度。
