Graves R A, Pandey N B, Chodchoy N, Marzluff W F
Cell. 1987 Feb 27;48(4):615-26. doi: 10.1016/0092-8674(87)90240-6.
When DNA synthesis is inhibited, the mRNAs coding for the replication-dependent histone proteins are selectively destabilized. The histone genes have been altered and reintroduced into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene. Two features of the mRNA are necessary for regulation of degradation: first, the hairpin loop must be present at the 3' end of the histone mRNA; and second, the histone mRNA must be capable of being translated to within 300 nucleotides of the 3' end of the RNA. Polyadenylated histone mRNAs are stable, as are histone mRNAs that contain in-frame termination codons early in the coding region or 500 nucleotide 3' untranslated regions with a normal hairpin loop at the 3' end.
当DNA合成受到抑制时，编码依赖复制的组蛋白的mRNA会被选择性地降解。通过与疱疹病毒胸苷激酶基因共转染，组蛋白基因已被改变并重新导入tk-小鼠L细胞。mRNA的两个特征对于降解调控是必需的：首先，组蛋白mRNA的3'端必须存在发夹环；其次，组蛋白mRNA必须能够在RNA 3'端的300个核苷酸内进行翻译。多聚腺苷酸化的组蛋白mRNA是稳定的，编码区早期含有框内终止密码子或3'端带有正常发夹环的500个核苷酸3'非翻译区的组蛋白mRNA也是稳定的。
