Lynn T C, Wang J H, Yang C S, Tu S M
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1986 Aug;19(3):177-82.
In 1984 and 1985, a total of 30 patients with biopsy confirmed nasopharyngeal carcinoma (NPC) were randomly selected for studies on natural killer cell (NK) activity. Control study on 30 normal subjects were also done. Lymphocyte separation was done by centrifuge of heparinized peripheral blood with Ficoll-Hypaque and monocytes were removed by Petri dish adhesion. K562 cells were used as target cells of NK activity. The lymphocyte-target reaction of 4 hours was done and the ratio was 50:1, 25:1 and 12.5:1. The results of 50:1 was taken as the NK activity. The mean value of NK activity in NPC patients was 34.8 +/- 22.7%, this is significantly different (t = 2.90, p less than 0.01) from 51.6 +/- 22.1% of the controls. If NK activity of 20% or more are taken for the normal standard, then the positive rate in NPC patients was 63.3% and that in the controls was 90.0%. The difference is significant, as chi 2 = 4.56, p less than 0.05. The NK activity in NPC patients is not correlated with the sex and age of the patients, the disease extent and the EB virus associated antibody titers.
1984年和1985年，总共随机选取了30例经活检确诊的鼻咽癌（NPC）患者进行自然杀伤细胞（NK）活性研究。同时对30名正常受试者进行了对照研究。采用Ficoll-Hypaque对肝素化外周血进行离心分离淋巴细胞，并用培养皿黏附法去除单核细胞。以K562细胞作为NK活性的靶细胞。进行4小时的淋巴细胞-靶细胞反应，比例为50:1、25:1和12.5:1。以50:1的结果作为NK活性。NPC患者NK活性的平均值为34.8 +/- 22.7%，与对照组的51.6 +/- 22.1%相比有显著差异（t = 2.90，p < 0.01）。若以20%及以上的NK活性作为正常标准，则NPC患者的阳性率为63.3%，对照组为90.0%。差异具有显著性，χ2 = 4.56，p < 0.05。NPC患者的NK活性与患者的性别、年龄、疾病范围及EB病毒相关抗体滴度无关。
