Lynn T C, Yang C S, Huang S C, Tu S M
Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, ROC.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1988 Feb;21(1):75-8.
From 1984 through 1986, a total of 20 patients with biopsy confirmed nasopharyngeal carcinoma (NPC) were randomly selected for studies on natural killer cell (NK) activity before and at the end of radiotherapy. Lymphocyte separation was done by centrifuge of heparinized peripheral blood with Ficoll-Hypaque and monocytes were removed by Petri dish adhesion. K562 cells were used as target cells of NK activity. The lymphocyte-target reaction of 4 hours was done and the ratio was 50:1, 25:1, and 12.5:1. The results of 50:1 was taken as the NK activity. The mean value of NK activity in NPC patients after radiotherapy was 43.0 +/- 21.1%, this is not significantly different from 41.2 +/- 23.6% before radiotherapy. On the other hand, before radiotherapy, the patients with too high NK activity (greater than 40%) or too low NK activity (less than 20%) had a mild tendency toward median value (20%-40%) after radiotherapy.
从1984年到1986年，共有20例经活检确诊为鼻咽癌（NPC）的患者被随机挑选出来，用于研究放疗前及放疗结束时的自然杀伤细胞（NK）活性。通过用Ficoll-Hypaque对肝素化外周血进行离心来分离淋巴细胞，并用培养皿黏附法去除单核细胞。将K562细胞用作NK活性的靶细胞。进行4小时的淋巴细胞-靶细胞反应，比例分别为50:1、25:1和12.5:1。将50:1时的结果作为NK活性。放疗后NPC患者的NK活性平均值为43.0 +/- 21.1%，与放疗前的41.2 +/- 23.6%无显著差异。另一方面，放疗前NK活性过高（大于40%）或过低（小于20%）的患者在放疗后有向中位值（20%-40%）靠拢的轻微趋势。
