Lynn T C, Hsieh R P, Tu S M
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1985 May;18(2):70-8.
Lymphocyte subsets and Epstein-Barr virus (EBV)-associated antibodies were studied in 108 patients with nasopharyngeal carcinoma (NPC) and in 34 normal controls. Lymphocyte subsets were identified with monoclonal antibodies (Ortho Co.) by indirect immunofluorescent antibody (IFA) method. The helper T lymphocytes (OKT4+) in NPC patients comprised 38.2 +/- 10.5% which is significantly different from 45.2 +/- 8.0% in controls. The helper/suppressor ratio in NPC patients was 1.33 +/- 0.65 which is significantly different from 1.64 +/- 0.48 in controls, but the ratio was not correlated with disease extent, sex, age, total lymphocyte counts, WBC counts and EBV-associated antibodies of NPC patients. There were no remarkable differences between NPC patients and controls in B lymphocytes (OKIa+), total T lymphocytes (OKT3+), and suppressor lymphocyte (OKT8+) percentages, total lymphocyte counts and WBC counts. The EBV-associated antibodies were titrated by the IFA method using P3HR-1 cells and Raji cells induced by IUdR as target. Mean antibody titers and seropositive rates showed significant increase in NPC patients (1:12-1:502 and 45.4%-68.5%, respectively) compared with controls (1:1-1:87 and 1.0%-5.9%, respectively). The increase in antibodies was positively correlated with NPC disease extent, but was not correlated with the sex, age, total lymphocyte counts, helper/suppressor ratio, and WBC counts of NPC patients.
对108例鼻咽癌患者和34例正常对照者进行了淋巴细胞亚群及爱泼斯坦-巴尔病毒（EBV）相关抗体的研究。采用间接免疫荧光抗体（IFA）法，用单克隆抗体（Ortho公司）鉴定淋巴细胞亚群。鼻咽癌患者的辅助性T淋巴细胞（OKT4+）占38.2±10.5%，与对照组的45.2±8.0%有显著差异。鼻咽癌患者的辅助/抑制细胞比值为1.33±0.65，与对照组的1.64±0.48有显著差异，但该比值与鼻咽癌患者的疾病范围、性别、年龄、淋巴细胞总数、白细胞计数及EBV相关抗体无关。鼻咽癌患者与对照组在B淋巴细胞（OKIa+）、总T淋巴细胞（OKT3+）、抑制性淋巴细胞（OKT8+）百分比、淋巴细胞总数及白细胞计数方面无显著差异。采用IFA法，以经碘脱氧尿苷（IUdR）诱导的P3HR-1细胞和Raji细胞为靶细胞，滴定EBV相关抗体。与对照组（分别为1:1 - 1:87和1.0% - 5.9%）相比，鼻咽癌患者的平均抗体滴度和血清阳性率显著升高（分别为1:12 - 1:502和45.4% - 68.5%）。抗体升高与鼻咽癌疾病范围呈正相关，但与鼻咽癌患者的性别、年龄、淋巴细胞总数、辅助/抑制细胞比值及白细胞计数无关。
