Universidad de Almería, Departamento de Psicología, Ctra. Sacramento S/N, 04120, La Cañada de San Urbano, Almería, Spain; Achucarro, Basque Center for Neuroscience Science Park, edificio de la Sede UPV/EHU, 48940 Leioa, Spain.
Universidad Miguel Hernández de Elche, Unidad de Neuroprótesis y Rehabilitación Visual, Av. de la Universidad S/N, Elche, Alicante, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
Brain Res. 2019 Feb 1;1704:94-102. doi: 10.1016/j.brainres.2018.09.039. Epub 2018 Oct 2.
In this work visual functional improvement of amblyopic Long Evans rats treated with tDCS has been assessed using the "slow angled-descent forepaw grasping" (SLAG) test. This test is based on an innate response that does not requires any memory-learning component and has been used before for measuring visual function in rodents. The results obtained show that this procedure is useful to assess monocular but not binocular deficits, as controls and amblyopic animals showed significant differences during monocular but not during binocular assessment. On the other hand, parvoalbumin labelling was analysed in three areas of the visual cortex (V1M, V1B and V2L) before and after tDCS treatment. No changes in labelling were observed after monocular deprivation. However, tDCS treatment significantly improved vision through the amblyopic eye, and a significant increase of parvoalbumin-positive cells was observed in the three areas, both in the stimulated hemisphere but also in the non-stimulated hemisphere. This effect occurred both in control and amblyopic animals. Thus, tDCS induced changes are similar in controls and amblyopic animals, although only the last one showed a functional improvement.
本工作采用“慢斜降前爪抓握”（SLAG）测试评估经 tDCS 治疗的弱视 Long Evans 大鼠的视觉功能改善。该测试基于一种先天反应，不依赖任何记忆学习成分，以前曾用于测量啮齿动物的视觉功能。结果表明，该程序可用于评估单眼但不能评估双眼缺陷，因为对照组和弱视动物在单眼评估时表现出显著差异，但在双眼评估时没有差异。另一方面，在 tDCS 治疗前后分析了三个视皮层区域（V1M、V1B 和 V2L）的 parvoalbumin 标记。单眼剥夺后未见标记变化。然而，tDCS 治疗通过弱视眼显著改善了视力，在刺激半球和非刺激半球的三个区域都观察到 parvoalbumin 阳性细胞的显著增加。这种效应发生在对照组和弱视动物中。因此，tDCS 诱导的变化在对照组和弱视动物中相似，尽管只有后者显示出功能改善。
