中华合附蚊（双翅目：蚋科）线粒体基因组全序列特征及其系统发育分析。

Characterization of the complete mitochondrial genome of Simulium (Byssodon) maculatum (Diptera: Simuliidae) and its phylogenetic implications.

机构信息

Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China.

出版信息

Int J Biol Macromol. 2019 Jan;121:152-160. doi: 10.1016/j.ijbiomac.2018.09.205. Epub 2018 Oct 1.

DOI:10.1016/j.ijbiomac.2018.09.205

PMID:30287375

Abstract

The mitochondrial (mt) genome of the black fly Simulium (Byssodon) maculatum, a pest of great importance for both humans and livestock, is sequenced and annotated for the first time. The genome structure, gene order and codon usage are typical among Diptera mt genomes. The mt genome is circular and 15,799 bp in length with 13 protein coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (CR), and with weakly positive AT-skew (0.02) and negative GC-skew (-0.12). Phylogenetic relationships of 16 species representing five families of Culicomorpha and two outgroups, based on mt genome data, were analyzed using both Maximum Likelihood and Bayesian methods. The monophyly of Culicomorpha is well supported, while Chironomoidea is indicated as a paraphyletic group. The well supported monophyletic Simuliidae is the sister group to Culicidae.

摘要

黑蝇 Simulium (Byssodon) maculatum 的线粒体（mt）基因组首次被测序并注释，该黑蝇对人类和牲畜都具有重要的危害性。基因组结构、基因顺序和密码子使用情况在双翅目 mt 基因组中具有典型特征。mt 基因组是圆形的，长度为 15799bp，包含 13 个蛋白质编码基因（PCGs）、22 个转移 RNA 基因（tRNAs）、两个核糖体 RNA 基因（rRNAs）和一个控制区（CR），具有较弱的正 AT 倾斜（0.02）和负 GC 倾斜（-0.12）。基于 mt 基因组数据，使用最大似然法和贝叶斯法对代表 Culicomorpha 五个科和两个外群的 16 个物种的系统发育关系进行了分析。Culicomorpha 的单系性得到很好的支持，而 Chironomoidea 则被表明为一个并系群。得到很好支持的单系 Simuliidae 是 Culicidae 的姐妹群。