Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Zhongke Research Institute of Industrial Technology, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang City, 438000, Hubei, People's Republic of China.
College of Life Sciences, Nankai University, Tianjin, 300071, China.
Sci Rep. 2023 Jun 6;13(1):9200. doi: 10.1038/s41598-023-36227-9.
The family Chironomidae is represented by seven subfamilies in China, among which Chironominae and Orthocladiinae are the most diverse. To gain a better understanding of the architecture and evolution of the mitogenomes of Chironomidae, we sequenced mitogenomes of twelve species (including two published species) of the two subfamilies Chironominae and Orthocladiinae, and comparative mitogenomic analyses were performed. Thus, we identified highly conserved genome organization of twelve species with regard to genome content, nucleotide and amino acid composition, codon usage, and gene characteristics. The K/K values of most protein-coding genes were far smaller than 1, indicating that these genes were evolving under purifying selection. Phylogenetic relationships between the family Chironomidae were reconstructed using 23 species representing six subfamilies, based on protein-coding genes and rRNAs using Bayesian Inference and Maximum Likelihood. Our results suggested the following relationship within the Chironomidae: (Podonominae + Tanypodinae) + (Diamesinae + (Prodiamesinae + (Orthocladiinae + Chironominae))). This study contributes to the mitogenomic database of Chironomidae, which will be significant for studing the mitogenome evolution of Chironomidae.
摇蚊科在中国有 7 个亚科，其中摇蚊亚科和直翅目科最为多样化。为了更好地了解摇蚊科的线粒体基因组结构和进化，我们对 2 个亚科摇蚊亚科和直翅目科的 12 种（包括 2 种已发表的种）进行了测序，并进行了比较线粒体基因组分析。因此，我们确定了 12 个物种的高度保守的基因组组织，包括基因组含量、核苷酸和氨基酸组成、密码子使用和基因特征。大多数蛋白质编码基因的 K/K 值远小于 1，表明这些基因在进化过程中受到了纯化选择的影响。基于代表 6 个亚科的 23 个物种的蛋白质编码基因和 rRNA，使用贝叶斯推断和最大似然法重建了摇蚊科的系统发育关系。我们的结果表明，摇蚊科内部的关系如下：（Podonominae+Tanypodinae）+（Diamesinae+（Prodiamesinae+（Orthocladiinae+Chironominae）））。本研究为摇蚊科的线粒体基因组数据库做出了贡献，这将对研究摇蚊科的线粒体基因组进化具有重要意义。
