Rotola A, Di Luca D, Monini P, Manservigi R, Tognon M, Virgili A, Segala V, Trapella G, Cassai E
Eur J Cancer Clin Oncol. 1986 Oct;22(10):1259-65. doi: 10.1016/0277-5379(86)90329-9.
DNA sequences homologous to HSV-1 and HSV-2 DNA fragments were searched in 64 genital, 35 labial and 34 cerebral tumors. Southern blot transfers of tumor and control DNAs were hybridized in stringent conditions with 32P labelled probes from HSV-1 and HSV-2 cloned DNA fragments. Specific hybridization to HSV-2 BglII N fragment was observed in six (9.4%) genital tumors. Labial and cerebral tumors did not show hybridization to any of the probes used. The technique employed allowed the detection of 0.1 copies of viral fragments per diploid genome.
在64例生殖器肿瘤、35例唇肿瘤和34例脑肿瘤中搜索与单纯疱疹病毒1型（HSV - 1）和单纯疱疹病毒2型（HSV - 2）DNA片段同源的DNA序列。肿瘤和对照DNA的Southern印迹转移在严格条件下与来自HSV - 1和HSV - 2克隆DNA片段的32P标记探针杂交。在6例（9.4%）生殖器肿瘤中观察到与HSV - 2 BglII N片段的特异性杂交。唇肿瘤和脑肿瘤未显示与所用任何探针的杂交。所采用的技术能够检测到每个二倍体基因组中0.1份病毒片段。
