Quan Xiao-Tian, Liu Qing-Zhen, Siddiqi Muhammad Zubair, Im Wan-Taek
Department of Biological Sciences, School of Life Sciences, Nantong University, 9 Seyuan-lu, Nantong, 226-019, Jiangsu, People's Republic of China.
Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Gyeonggi-do, 17579, Republic of Korea.
Arch Microbiol. 2019 May;201(4):443-449. doi: 10.1007/s00203-018-1577-0. Epub 2018 Oct 4.
A Gram-stain-negative, strictly aerobic, non-motile, ivory colored and rod-shaped bacterium (designated Gsoil 652) isolated from ginseng cultivating soil, was characterized using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 652 was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Phylogenetic analysis, based on 16S rRNA gene sequences similarities, indicated that Gsoil 652 belongs to the genus Caballeronia of the family Burkholderiaceae and was most closely related to Caballeronia choica LMG 22940 (98.9%), Caballeronia udeis LMG 27134 (98.9%), Caballeronia sordidicola LMG 22029 (98.2%) and Caballeronia humi LMG 22934 (98.1%). The DNA G+C content was 62.1 mol% and Q-8 was the major isoprenoid quinone. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid, and unidentified phospholipid. The predominant fatty acids were C, C cyclo and C cyclo ω8c. The DNA-DNA relatedness value between strain Gsoil 652 and closely related type strains of Caballeronia species were less than 36.0%. Moreover, strain Gsoil 652 could be distinguished phenotypically from the recognized species of the genus Caballeronia. The novel isolate, therefore, represents a novel species, for which the name Caballeronia ginsengisoli sp. nov. is proposed, with the type strain Gsoil 652 (= KACC 19441 = LMG 30326).
从人参种植土壤中分离出一株革兰氏染色阴性、严格需氧、无运动性、象牙色的杆状细菌（命名为Gsoil 652），采用多相分类法对其进行特征分析以明确其分类地位。观察到菌株Gsoil 652在R2A琼脂培养基上于30℃和pH 7.0条件下生长最佳。基于16S rRNA基因序列相似性的系统发育分析表明，Gsoil 652属于伯克霍尔德氏菌科的卡瓦勒罗尼亚属，与卡瓦勒罗尼亚乔伊卡LMG 22940（98.9%）、卡瓦勒罗尼亚乌代斯LMG 27134（98.9%）、卡瓦勒罗尼亚污栖菌LMG 22029（98.2%）和卡瓦勒罗尼亚湿地菌LMG 22934（98.1%）关系最为密切。DNA G+C含量为62.1 mol%，Q-8是主要的类异戊二烯醌。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、未鉴定的氨基磷脂和未鉴定的磷脂。主要脂肪酸为C、C环和C环ω8c。菌株Gsoil 652与卡瓦勒罗尼亚属相关模式菌株之间的DNA-DNA相关性值小于36.0%。此外，菌株Gsoil 652在表型上可与卡瓦勒罗尼亚属已确认的物种区分开来。因此，该新分离菌株代表一个新物种，提议将其命名为卡瓦勒罗尼亚人参分离菌新种，模式菌株为Gsoil 652（=KACC 19441=LMG 30326）。
