利用无虹膜-威尔姆斯瘤相关缺失对11号染色体短臂上的DNA探针进行区域定位。

Regional localization of DNA probes on the short arm of chromosome 11 using aniridia-Wilms' tumor-associated deletions.

作者信息

Mannens M, Slater R M, Heyting C, Geurts van Kessel A, Goedde-Salz E, Frants R R, Van Ommen G J, Pearson P L

出版信息

Hum Genet. 1987 Feb;75(2):180-7. doi: 10.1007/BF00591083.

Abstract

We are interested in the precise localization of various DNA probes on the short arm of chromosome 11 for our research on the aniridia-Wilms' tumor association (AWTA), assigned to region 11p13 (Knudson and Strong 1972; Riccardi et al. 1978). For this purpose we have screened lymphocyte DNA and material derived from somatic cell hybrids from individuals with constitutional 11p deletions with a range of available probes: D11S12; calcitonin/CGRP (CALC1/CALC2); insulin (INS); Harvey ras 1 (HRAS 1); beta-globin gene cluster (HBBC); human insulin-like growth factor 2 (IGF-2); parathyroid hormone (PTH); human pepsinogen A (PGA). Using this material, it has been possible to map all probes used, except insulin, outside the region 11p111-p15.1, resulting in an SRO (same regional overlap) of 11p15.1-p15.5 for most probes. We found an SRO for PGA of 11p111-q12 and an SRO for CALC2 of 11p15.1-p15.5 or 11p111-q12. We have localised the insulin gene to band 11p15.1.

摘要

我们对11号染色体短臂上各种DNA探针的精确定位感兴趣，以便进行我们关于无虹膜-威尔姆斯瘤关联（AWTA）的研究，该关联被定位于11p13区域（Knudson和Strong，1972年；Riccardi等人，1978年）。为此，我们用一系列可用探针筛选了淋巴细胞DNA以及来自11号染色体短臂先天性缺失个体的体细胞杂种材料：D11S12；降钙素/CGRP（CALC1/CALC2）；胰岛素（INS）；哈维鼠肉瘤病毒癌基因1（HRAS 1）；β-珠蛋白基因簇（HBBC）；人胰岛素样生长因子2（IGF-2）；甲状旁腺激素（PTH）；人胃蛋白酶原A（PGA）。利用这些材料，除胰岛素外，已将所有使用的探针定位在11p111-p15.1区域之外，大多数探针的最小重叠区域（SRO）为11p15.1-p15.5。我们发现PGA的SRO为11p111-q12，CALC2的SRO为11p15.1-p15.5或11p111-q12。我们已将胰岛素基因定位到11p15.1带。