Harper M E, Ullrich A, Saunders G F
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4458-60. doi: 10.1073/pnas.78.7.4458.
The human insulin gene was mapped by hybridization in situ of 3H-labeled recombinant plasmid pHIG900 to chromosome preparations in the presence of 10% dextran sulfate. pHIG900 contains a 900-base pair insert of human genomic DNA that includes the coding region for the C peptide and intervening sequence 2. A significant percentage of hybridized cells (34%) exhibited silver grains on the distal end of the short arm (band p15) of chromosome 11. Furthermore, silver grains on this region represented 26% of all chromosomal label. These results demonstrate chromosomal localization of the human insulin gene to 11p15.
在10%硫酸葡聚糖存在的情况下，通过将3H标记的重组质粒pHIG900与染色体标本进行原位杂交，对人类胰岛素基因进行了定位。pHIG900含有一段900个碱基对的人类基因组DNA插入片段，其中包括C肽的编码区和间隔序列2。相当比例（34%）的杂交细胞在11号染色体短臂（p15带）的远端显示出银颗粒。此外，该区域的银颗粒占所有染色体标记的26%。这些结果证明人类胰岛素基因定位于11p15。
