a Division of Infectious Diseases, Rhode Island Hospital , Warren Alpert Medical School at Brown University , Providence , RI , USA.
b Mycology Laboratory, Division of Infectious Diseases , Wadsworth Center, New York State Department of Health , Albany , NY , USA.
Virulence. 2018;9(1):1539-1547. doi: 10.1080/21505594.2018.1518087.
Laboratory investigations of the pathogenesis of Pseudogymnoascus destructans, the fungal causal agent of bat White Nose Syndrome (WNS), presents unique challenges due to its growth requirements (4°-15°C) and a lack of infectivity in the current disease models. Pseudogymnoascus pannorum is the nearest fungal relative of P. destructans with wider psychrophilic - physiological growth range, and ability to cause rare skin infections in humans. Our broad objectives are to create the molecular toolkit for comparative study of P. destructans and P. pannorum pathogenesis. Towards these goals, we report the successful development of an invertebrate model in the greater wax moth Galleria mellonella. Both P. destructans and P. pannorum caused fatal disease in G. mellonella and elicited immune responses and histopathological changes consistent with the experimental disease.
由于其生长要求（4°C-15°C）以及当前疾病模型中缺乏感染力，因此对假丝酵母菌的发病机制进行实验室研究具有独特的挑战。假丝酵母菌是假丝酵母菌的最近真菌亲缘种，具有更广泛的嗜冷生理生长范围，并且能够引起人类罕见的皮肤感染。我们的总体目标是为假丝酵母菌和假丝酵母菌发病机制的比较研究创建分子工具包。为此，我们报告了在大蜡螟中成功建立了一种无脊椎动物模型。假丝酵母菌和假丝酵母菌都导致大蜡螟致命疾病，并引起免疫反应和组织病理学变化，与实验疾病一致。
