Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710054, China.
Department of Biomedical Engineering, Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, Michigan, 48109-1078.
J Biomed Mater Res A. 2018 Dec;106(12):3175-3184. doi: 10.1002/jbm.a.36512. Epub 2018 Oct 5.
Development of biodegradable and highly biocompatible polymer with intrinsical photoluminescence and high photostability for real-time live cell bioimaging has attracted much attention recently. Here, a biodegradable and amphiphilic poly (citrate)-co-poly (ethylene glycol) (PEG) grafted with arginine (PCGA) polymer with intrinsical fluorescence was synthesized for targeted live cell bioimaging. The physicochemical structure, photoluminescent properties, hemocompatibility, cytotoxicity, and fluorescent bioimaging studies in live cells were determined in detail. PCGA showed a significantly high hemocompatibility, low cytotoxicity, and excellent photostability, which allows for imaging the live cells attachment and proliferation. Furthermore, PCGA could efficiently enhance cell attachment and proliferation due to the presence of arginine, suggesting their high cellular biocompatibility. Importantly, PCGA could selectively stain the lysosome in cells. Our results demonstrated that the amino acid-based polymer functionalization may be an important strategy to develop multifunctional biomaterials with enhanced biocompatibility for targeted bioimaging, cancer therapy, and regenerative medicine. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3175-3184, 2018.
最近，人们对开发具有内在光致发光和高光稳定性的可生物降解和高度生物相容的聚合物以用于实时活细胞生物成像引起了广泛关注。在这里，我们合成了一种具有内在荧光的可生物降解的两亲性聚（柠檬酸盐）-共-聚乙二醇（PEG）接枝精氨酸（PCGA）聚合物，用于靶向活细胞生物成像。详细确定了其物理化学结构、光致发光性能、血液相容性、细胞毒性和活细胞荧光生物成像研究。PCGA 表现出非常高的血液相容性、低细胞毒性和优异的光稳定性，允许对活细胞的附着和增殖进行成像。此外，由于存在精氨酸，PCGA 可以有效地增强细胞附着和增殖，表明其具有很高的细胞生物相容性。重要的是，PCGA 可以选择性地染色细胞中的溶酶体。我们的结果表明，基于氨基酸的聚合物功能化可能是开发具有增强生物相容性的多功能生物材料的重要策略，用于靶向生物成像、癌症治疗和再生医学。 © 2018 威利父子公司。生物医学材料研究杂志 A 部分：3175-3184，2018 年。
