D-丝氨酸脱氨酶控制区及结构基因N端部分的DNA序列
DNA sequences of the D-serine deaminase control region and N-terminal portion of the structural gene.
作者信息
McFall E, Runkel L
出版信息
J Bacteriol. 1983 Jun;154(3):1508-12. doi: 10.1128/jb.154.3.1508-1512.1983.
Abstract
We determined the DNA sequence of the D-serine deaminase promoter region and of the N-terminal region of the structural gene. There are possibilities in the promoter for secondary structure and for initiation recognition sequences, and there is an open reading frame. The N-terminal sequence for the structural gene confirms that part of the amino acid sequence previously determined by E. Schlitz and W. Schmitt (FEBS Lett. 134:57-62, 1981), including the active site of the enzyme, and spans the two regions unresolved by their work.
摘要
我们测定了D-丝氨酸脱氨酶启动子区域和结构基因N端区域的DNA序列。启动子中存在形成二级结构和起始识别序列的可能性，并且存在一个开放阅读框。结构基因的N端序列证实了先前由E.施利茨和W.施密特（《欧洲生物化学学会联合会快报》134:57 - 62，1981）确定的部分氨基酸序列，包括该酶的活性位点，并且跨越了他们工作中未解决的两个区域。
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