Bangladesh Jute Research Institute, Dhaka, 1207, Bangladesh; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
Plant Physiol Biochem. 2018 Nov;132:424-433. doi: 10.1016/j.plaphy.2018.09.020. Epub 2018 Sep 23.
Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is responsible for severe yield losses of up to 90% in all soybean producing countries. Till today, eight resistance to Phakopsora pachyrhizi (Rpp) loci have been mapped in soybean. Their resistance mechanism is race specific but largely unknown. The transcriptomes of susceptible BRS184 and Rpp3 with ASR isolates T1-2 at 24 h after inoculation (hai) and without ASR inoculation (mock) were annotated by similarity searching with different databases. A total of 4518 differentially expressed genes were identified. We found 70.89%, 56.61%, 32.13%, and 56.04% genes in the protein family databases (PFAM), Gene Ontology (GO), Eukaryotic clusters of Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG), respectively. KEGG disclosed that 52% of the phenylpropanoid pathway related genes were up-regulated. The relative gene expression study for selected genes of that pathway was conducted by RT-qPCR using Rpp1-Rpp4 carrying lines with T1-2 infection. The RT-qPCR results revealed that the Rpp lines utilized these genes in a rate limiting manner as a defence response. With the exception of glycinol 4-dimethylallyltransferase (G4DT) and chalcone reductase (CHR), all the genes showed the greatest expression at 12 hai, but the gene expressions which occur between 24 and 96 hai make these Rpp lines unique to their respective ASR isolates. Moreover, functional coordination of arogenate dehydratase 6 (ADT6) and 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (ispG), chalcone synthase (CHS) and CHR, and G4DT and phytyltransferase 3 (PT3) may have a great impact on soybean resistance against ASR.
亚洲大豆锈病（ASR）由专性活体营养真菌 Phakopsora pachyrhizi 引起，在所有大豆生产国中造成高达 90%的严重产量损失。迄今为止，已在大豆中定位了 8 个对 Phakopsora pachyrhizi（Rpp）的抗性基因座。它们的抗性机制是专化性的，但大部分尚不清楚。易感品种 BRS184 和具有 ASR 分离株 T1-2 的 Rpp3 的转录组在接种（hai）24 小时后和无 ASR 接种（mock）的情况下，通过与不同数据库的相似性搜索进行注释。共鉴定出 4518 个差异表达基因。我们在蛋白质家族数据库（PFAM）、基因本体论（GO）、真核同源群（KOG）和京都基因与基因组百科全书通路（KEGG）中分别发现了 70.89%、56.61%、32.13%和 56.04%的基因。KEGG 揭示了 52%的苯丙烷途径相关基因上调。通过 RT-qPCR 对携带 T1-2 感染的 Rpp1-Rpp4 品系的该途径的选定基因进行相对基因表达研究。RT-qPCR 结果表明，Rpp 品系以限速方式利用这些基因作为防御反应。除甘氨酸 4-二甲基烯丙基转移酶（G4DT）和查尔酮还原酶（CHR）外，所有基因在 12 hai 时表达最大，但在 24 和 96 hai 之间发生的基因表达使这些 Rpp 品系对各自的 ASR 分离株具有独特性。此外，芳香酸脱水酶 6（ADT6）和 4-羟基-3-甲基丁-2-烯基二磷酸合酶（ispG）、查尔酮合酶（CHS）和 CHR，以及 G4DT 和植基转移酶 3（PT3）的功能协调可能对大豆抗 ASR 有很大影响。
