Okuda A, Sakai M, Muramatsu M
J Biol Chem. 1987 Mar 15;262(8):3858-63.
We have isolated the rat placental-type glutathione S-transferase (GST-P) gene from a lambda phage library using GST-P cDNA clone, pGP5 (Sugioka, Y., Kano, T., Okuda, A., Sakai, M., Kitagawa, T., and Muramatsu, M. (1985) Nucleic Acids Res. 13, 6049-6057), as a probe. The rat GST-P gene is about 3 kilobase pairs long and contains 7 exons and 6 introns, encoding the same GST-P protein specified by pGP5. The cap site maps 70 nucleotides upstream from the translation initiation site. The canonical promoter "TATA" box was found 27 base pairs upstream from the putative cap site. Two hundred base pairs upstream from the cap site are rich in G + C residues (61%), and the hexanucleotide sequence 5'-GGGCGG-3' is found at position -47 to -42. We have also isolated several processed-type pseudogenes which were presumably originated by reverse transcription followed by insertion at target sites.
我们使用谷胱甘肽S-转移酶P型（GST-P）cDNA克隆pGP5（杉冈洋、加纳彻、奥田明、酒井真、北川彻和村松满，（1985年）《核酸研究》13卷，6049 - 6057页）作为探针，从λ噬菌体文库中分离出大鼠胎盘型谷胱甘肽S-转移酶（GST-P）基因。大鼠GST-P基因约3千碱基对长，包含7个外显子和6个内含子，编码与pGP5所指定的相同的GST-P蛋白。帽位点位于翻译起始位点上游70个核苷酸处。在假定的帽位点上游27个碱基对处发现了典型的启动子“TATA”框。帽位点上游200个碱基对富含G + C残基（61%），并且在-47至-42位发现了六核苷酸序列5'-GGGCGG-3'。我们还分离出了几个加工型假基因，推测它们是由逆转录随后插入靶位点产生的。
